Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.
Program of Petrochemical and Polymer Science, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.
Carbohydr Polym. 2012 Sep 1;90(1):73-7. doi: 10.1016/j.carbpol.2012.04.064. Epub 2012 May 8.
Hydrolysis of α-chitin in concentrated hydrochloric acid under elevated temperature is a general preparation of a nutrapharmaceutical glucosamine hydrochloride (GlcN.HCl). In this study, the microwave and ultrasonic wave assisted acid hydrolysis of shrimp shell α-chitin are investigated with an aim to improve the reaction rate and selectivity. Microwave heating shortens the hydrolysis time from 120 min in the conventional heating process to 12 min to afford GlcN.HCl in 57% yield. Sonication can be used to assist chitin dissolution in 38% HCl prior to the hydrolysis at 120 °C for 120 min to produce GlcN.HCl in 62% yield. The selective hydrolysis of glycosidic bond of chitin is achievable at 30 °C for 4 h to give N-acetyl glucosamine (GlcNAc) in 37% yield.
在高温下用浓盐酸水解α-壳聚糖是制备营养药物盐酸氨基葡萄糖（GlcN.HCl）的常用方法。本研究采用微波和超声波辅助酸水解虾壳α-壳聚糖，旨在提高反应速率和选择性。微波加热将水解时间从传统加热过程中的 120 分钟缩短至 12 分钟，产率为 57%的 GlcN.HCl。超声处理可在 38%盐酸中辅助壳聚糖溶解，然后在 120°C 下水解 120 分钟，产率为 62%的 GlcN.HCl。在 30°C 下选择性水解糖苷键 4 小时可得到 37%产率的 N-乙酰氨基葡萄糖（GlcNAc）。
