嗜热四膜虫细胞质核糖体亚基中的蛋白质 - RNA交联。

Protein-RNA crosslinking in the subunits of the cytoplasmic ribosome of Tetrahymena thermophila.

作者信息

Petridou B, Guerin M F, Hayes F

机构信息

Laboratoire de Chimie Cellulaire, Institut de Biologie Physico-Chimique, Paris, France.

出版信息

Biochimie. 1989 May;71(5):667-79. doi: 10.1016/0300-9084(89)90161-2.

DOI:10.1016/0300-9084(89)90161-2

Abstract

Use of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide to introduce RNA-protein crosslinks in the 40S and 60S subunits of the cytoplasmic ribosome of Tetrahymena thermophila is described, and proteins linked covalently to 17S and 26S ribosomal RNAs are identified. RNA-protein crosslinking is accompanied by extensive dimerization and aggregation of ribosomal subunits probably due to formation of interparticle protein-protein crosslinks.

摘要

描述了使用1-乙基-3-(3-二甲基氨基丙基)碳二亚胺在嗜热四膜虫细胞质核糖体的40S和60S亚基中引入RNA-蛋白质交联，并且鉴定了与17S和26S核糖体RNA共价连接的蛋白质。RNA-蛋白质交联伴随着核糖体亚基的广泛二聚化和聚集，这可能是由于颗粒间蛋白质-蛋白质交联的形成。

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