Application of Grimelius argyrophil staining to the study of tumour ultrastructure. III. Effects of osmium tetroxide.

The results obtained in electron microscopy for Grimelius (1968) argyrophil staining by the en bloc staining of Vassallo and the staining on section of Håkanson were compared on tumoral and non-tumoral tissue samples and were found to lead to different results. Our earlier results for the en bloc staining method indicated that the fixative used in this process was important and that embedding in resin followed by staining of section weakened silver impregnation. We examined here the effects of OsO4 post-fixation combined with such embedding and found that staining was obtained within a shorter period and was more intense. This staining on section was not specific enough, since it was observed in secretory granules of non-argyrophil cells such as those containing somatostatin. In addition, this staining was not confined to specific peripheral zone as it occurred after en bloc staining; particularly in secretory granules with double structure including a core such as those containing glucagon in the pancreas or certain granules in tumoral carcinoid tissue. Consequently silver deposits were located both in the core and peripheral zone of such granules. Moreover, after OsO4 post-fixation, embedding in resin, and staining on section, argentaffin and argyrophil properties intermingle.