Quinn's Advantage受精培养液与199组织培养液用于卵母细胞体外成熟的比较。
Comparison of Quinn's Advantage fertilization medium and tissue culture medium 199 for in vitro maturation of oocytes.
作者信息
Lin Yu-Hung, Hwang Jiann-Loung, Huang Lee-Wen, Seow Kok-Min, Hsieh Bih-Chwen, Tzeng Chii-Ruey
机构信息
Department of Obstetrics and Gynecology, Shin Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan; School of Medicine, Fu Jen Catholic University, Taipei, Taiwan; Department of Obstetrics and Gynecology, National Taiwan University Hospital, Taipei, Taiwan; Department of Obstetrics and Gynecology, Taipei Medical University, Taipei, Taiwan.
Department of Obstetrics and Gynecology, Shin Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan; Department of Obstetrics and Gynecology, Taipei Medical University, Taipei, Taiwan.
出版信息
Taiwan J Obstet Gynecol. 2014 Mar;53(1):17-20. doi: 10.1016/j.tjog.2013.12.001.
OBJECTIVE
The purpose of the study was to compare the Quinn's Advantage fertilization medium (Q1) and the tissue culture medium 199 (TCM199) for in vitro maturation (IVM) of oocytes and ammonium production during IVM.
MATERIALS AND METHODS
The immature murine oocytes were randomly added into Q1 and TCM199. Ammonium concentrations were measured at the start and after 18 hours of IVM, and the mature oocytes were fertilized and cultured into blastocysts. The blastocysts were then stained for inner cell mass (ICM) and trophectoderm.
RESULTS
The maturation rate was higher in Q1 than in TCM199 (85.7% vs. 76.6%, p = 0.024). The fertilization and blastocyst rates were slightly higher in Q1, but not significant. Differential staining of the blastocysts showed slightly higher ICM ratio in the blastocysts derived from Q1. Mean ammonium concentrations in Q1 and TCM199 at Time 0 were 184.9 and 339.2 μg/dL, respectively (p = 0.05), and after 18 hours of IVM were 268.7 and 443.6 μg/dL, respectively (p = 0.045). Addition of ammonium chloride into Q1 adversely affects IVM.
CONCLUSION
Q1 is superior to TCM199 in terms of oocyte maturation, which may be due to lower ammonium concentration.
目的
本研究旨在比较奎因优势受精培养基（Q1）和199组织培养基（TCM199）用于卵母细胞体外成熟（IVM）及IVM过程中铵产生的情况。
材料与方法
将未成熟的小鼠卵母细胞随机加入Q1和TCM199中。在IVM开始时及18小时后测量铵浓度，将成熟卵母细胞受精并培养成囊胚。然后对囊胚进行内细胞团（ICM）和滋养外胚层染色。
结果
Q1中的成熟率高于TCM199（85.7%对76.6%，p = 0.024）。Q1中的受精率和囊胚率略高，但无显著差异。囊胚的差异染色显示，源自Q1的囊胚中ICM比例略高。Q1和TCM199在时间0时的平均铵浓度分别为184.9和339.2μg/dL（p = 0.05），IVM 18小时后分别为268.7和443.6μg/dL（p = 0.045）。向Q1中添加氯化铵会对IVM产生不利影响。
结论
在卵母细胞成熟方面，Q1优于TCM199，这可能是由于铵浓度较低。
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