营养成分和植物生长调节剂对糯米（水稻）愈伤组织诱导及植株再生的影响

Influence of nutrient composition and plant growth regulators on callus induction and plant regeneration in glutinous rice (Oryza sativa L.).

作者信息

Duangsee K, Bunnag S

出版信息

Pak J Biol Sci. 2014 Jan 1;17(1):98-103. doi: 10.3923/pjbs.2014.98.103.

DOI:10.3923/pjbs.2014.98.103

Abstract

The potential for callus induction and regeneration depends on nutrient composition and plant growth regulators. The aim of the present study was to investigate the effect of nutrient composition and plant growth regulators on callus induction and plant regeneration in the glutinous rice cultivar Khunvang. The effect of 2,4-D concentrations (1, 2, 3, 4 and 5 mg L(-1)) on callus induction and growth were investigated. The results revealed that the highest percentage of callus induction (97%) was observed in MS medium supplemented with 5 mg L(-1) 2,4-D under 16 h Photoperiod. The effects of casein hydrolysate concentrations of casein hydrolysate (0, 300, 500, 700 and 900 mg L(-1)) and proline (0, 300, 500, 700 and 900 mg L(-1)) on callus induction and growth of Khunvang were also observed. The results indicated that the increasing casein hydrolysate and proline concentrations did not show a significant effect on callus growth. However, proline concentration of 900 mg L(-1) yielded 85.67% of callus growth.

摘要

愈伤组织诱导和再生的潜力取决于营养成分和植物生长调节剂。本研究的目的是调查营养成分和植物生长调节剂对糯米品种坤旺愈伤组织诱导和植株再生的影响。研究了2,4 - D浓度（1、2、3、4和5 mg L(-1)）对愈伤组织诱导和生长的影响。结果表明，在16小时光周期下，添加5 mg L(-1) 2,4 - D的MS培养基中观察到最高的愈伤组织诱导率（97%）。还观察了水解酪蛋白浓度（0、300、500、700和900 mg L(-1)）和脯氨酸（0、300、500、700和900 mg L(-1)）对坤旺愈伤组织诱导和生长的影响。结果表明，水解酪蛋白和脯氨酸浓度的增加对愈伤组织生长没有显著影响。然而，900 mg L(-1)的脯氨酸浓度产生了85.67%的愈伤组织生长率。