Volk R, Köster M, Pöting A, Hartmann L, Knöchel W
Institut für Molekularbiologie und Biochemie, Freie Universität Berlin, FRG.
EMBO J. 1989 Oct;8(10):2983-8. doi: 10.1002/j.1460-2075.1989.tb08448.x.
Screening of a Xenopus laevis oocyte cDNA library with a rat basic fibroblast growth factor (bFGF) cDNA led to the isolation of a 1.35 kb sequence containing exon III of the bFGF gene. Reverse complementary listing of this sequence revealed a polyadenylated transcript with an open reading frame coding for an unknown protein of mol. wt 24,292 daltons. The coding part of bFGF exon III is located in this putative mRNA in opposite direction within the 3' untranslated region. By hybridization studies on transcription orientation with single-stranded probes it could be proven that this transcript actually represents an antisense transcript to part of the Xenopus bFGF gene. Sequence organization on corresponding genomic fragments revealed that it is processed from a larger precursor by splicing mechanisms. Sequence comparison with elongated transcripts from the bFGF gene in human hepatoma has shown that the gene coding for the antisense mRNA is evolutionarily conserved.
用大鼠碱性成纤维细胞生长因子（bFGF）cDNA筛选非洲爪蟾卵母细胞cDNA文库，得到一个1.35 kb的序列，其中包含bFGF基因的外显子III。该序列的反向互补排列显示出一个聚腺苷酸化转录本，其开放阅读框编码一种分子量为24,292道尔顿的未知蛋白质。bFGF外显子III的编码部分位于该假定mRNA中，方向相反，位于3'非翻译区内。通过用单链探针进行转录方向的杂交研究，可以证明该转录本实际上代表非洲爪蟾bFGF基因部分的反义转录本。对相应基因组片段的序列分析表明，它是通过剪接机制从一个更大的前体加工而来的。与人类肝癌中bFGF基因延长转录本的序列比较表明，编码反义mRNA的基因在进化上是保守的。
