Orlov S N, Postnov I Y, Pokudin N I, Kukharenko V Y, Postnov Y V
Central Research Laboratory, Ministry of Public Health, Moscow, USSR.
J Hypertens. 1989 Oct;7(10):781-8. doi: 10.1097/00004872-198910000-00003.
The activity of ion-transport systems and Ca2+-induced erythrocyte haemolysis were compared between patients with essential hypertension and two strains of spontaneously hypertensive rats. Previous data on the increased rate of Na+-Li+ countertransport in erythrocytes of essential hypertensives were confirmed in this study. However, identification of Na+-Li+ countertransport in rat erythrocytes remained a complicated person because of the high rate of sodium-independent efflux of Li+. The rate of Na+-H+ exchange increased by 50-80% both in spontaneously hypertensive Wistar-Kyoto rats (SHR) and in patients with essential hypertension. No difference between Milan hypertensive strain rats (MHS) and Milan normotensive strain rats (MNS) was found. The rate of Na+,K+ cotransport increased in SHR and MHS erythrocytes compared with rats of the control strains [normotensive Wistar-Kyoto rats (WKY) and MNS; 30-50 and 90-110%, respectively]. No difference in this parameter was found between patients with essential hypertension and healthy subjects. Erythrocytes of patients with essential hypertension and of SHR were characterized by a higher sensitivity of their K+ channels to the increased concentration of intracellular Ca2+. This parameter did not change in MHS erythrocytes. Ca2+-induced haemolysis increased four- to fivefold in MHS erythrocytes compared with MNS and did not change in erythrocytes of SHR and patients with essential hypertension. The conclusion from these data is that the SHR strain is a more adequate model of human essential hypertension than the MHS.
比较了原发性高血压患者与两种自发性高血压大鼠品系的离子转运系统活性和钙离子诱导的红细胞溶血情况。本研究证实了先前关于原发性高血压患者红细胞中钠-锂逆向转运速率增加的数据。然而，由于锂离子非钠依赖性外流速率较高，大鼠红细胞中钠-锂逆向转运的鉴定仍然是一个复杂的问题。自发性高血压Wistar-Kyoto大鼠（SHR）和原发性高血压患者的钠-氢交换速率均增加了50%-80%。未发现米兰高血压品系大鼠（MHS）和米兰正常血压品系大鼠（MNS）之间存在差异。与对照品系大鼠[正常血压Wistar-Kyoto大鼠（WKY）和MNS]相比，SHR和MHS红细胞中的钠-钾协同转运速率增加，分别增加了30%-50%和90%-110%。原发性高血压患者与健康受试者在该参数上未发现差异。原发性高血压患者和SHR的红细胞的钾通道对细胞内钙离子浓度增加具有更高的敏感性。MHS红细胞中的这一参数没有变化。与MNS相比，MHS红细胞中钙离子诱导的溶血增加了四到五倍，而SHR和原发性高血压患者的红细胞中则没有变化。这些数据得出的结论是，SHR品系比MHS更适合作为人类原发性高血压的模型。
