Hassan Naglaa, Shimizu Masafumi, Hyakumachi Mitsuro
Department of Plant Pathology, Faculty of Agriculture, South Valley University, Qena 83523, Egypt. ; Laboratory of Plant Pathology, Faculty of Applied Biological Science, Gifu University, Gifu 501-1193, Japan.
Laboratory of Plant Pathology, Faculty of Applied Biological Science, Gifu University, Gifu 501-1193, Japan.
Mycobiology. 2014 Mar;42(1):66-72. doi: 10.5941/MYCO.2014.42.1.66. Epub 2014 Mar 31.
Roselle (Hibiscus sabdariffa L.) family Malvaceae is an important crop used in food, cosmetics and pharmaceutics industries. Roselle is cultivated mainly in Upper Egypt (Qena and Aswan governorates) producing 94% of total production. Root rot disease of roselle is one of the most important diseases that attack both seedlings and adult plants causing serious losses in crop productivity and quality. The main objective of the present study is to identify and characterize pathogens associated with root rot and wilt symptoms of roselle in Qena, Upper Egypt and evaluate their pathogenicity under greenhouse and field condition. Fusarium oxysporum, Macrophomina phaseolina, Fusarium solani, Fusarium equiseti and Fusarium semitectum were isolated from the natural root rot diseases in roselle. All isolated fungi were morphologically characterized and varied in their pathogenic potentialities. They could attack roselle plants causing damping-off and root rot/wilt diseases in different pathogenicity tests. The highest pathogenicity was caused by F. oxysporum and M. phaseolina followed by F. solani. The least pathogenic fungi were F. equiseti followed by F. semitectum. It obviously noted that Baladi roselle cultivar was more susceptible to infection with all tested fungi than Sobhia 17 under greenhouse and field conditions. This is the first report of fungal pathogens causing root rot and vascular wilt in roselle in Upper Egypt.
玫瑰茄（Hibiscus sabdariffa L.）属锦葵科，是一种在食品、化妆品和制药行业中使用的重要作物。玫瑰茄主要种植在上埃及（基纳省和阿斯旺省），产量占总产量的94%。玫瑰茄根腐病是侵袭幼苗和成年植株的最重要病害之一，会导致作物产量和品质严重损失。本研究的主要目的是鉴定和表征与上埃及基纳省玫瑰茄根腐病和枯萎症状相关的病原体，并在温室和田间条件下评估它们的致病性。尖孢镰刀菌、菜豆壳球孢、茄腐镰刀菌、木贼镰刀菌和半裸镰刀菌是从玫瑰茄自然根腐病中分离出来的。所有分离出的真菌都进行了形态学表征，其致病潜力各不相同。在不同的致病性试验中，它们会侵袭玫瑰茄植株，导致猝倒病和根腐病/枯萎病。致病性最强的是尖孢镰刀菌和菜豆壳球孢，其次是茄腐镰刀菌。致病性最弱的真菌是木贼镰刀菌，其次是半裸镰刀菌。明显可以看出，在温室和田间条件下，巴拉迪玫瑰茄品种比索布希亚17号更容易受到所有测试真菌感染。这是上埃及玫瑰茄中引起根腐病和维管束枯萎病的真菌病原体的首次报道。
