Kawase I, Shirasaka T, Ikeda T, Hara H, Tanio Y, Watanabe M, Saito S, Masuno T, Kishimoto S, Yamamura Y
Third Department of Internal Medicine, Osaka University Medical School.
Jpn J Cancer Res. 1989 Nov;80(11):1098-105. doi: 10.1111/j.1349-7006.1989.tb02265.x.
Four-hour exposure of C3H/HeN mouse spleen cells to Nocardia rubra cell wall skeleton (N-CWS) before 4-day culture with a suboptimal dose of human recombinant interleukin 2 (rIL 2) augmented the induction of lymphokine-activated killer (LAK) cell activity, whereas the treatment with N-CWS alone induced no cytotoxicity. In accordance with this, the IL 2 binding activity of spleen cells was augmented by combined stimulation with N-CWS and rIL 2. The augmented cytotoxicity was mediated by Thy-1.2+, Lyt-1.1-, Lyt-2.1- and asialo GM1+ cells. Cell cultures in diffusion chambers revealed that N-CWS-treated spleen cells produced a LAK cell induction-helper factor (LAK-helper factor, LHF) when cultured with rIL 2. The LHF production required Thy-1.2+, Lyt-1.1+, Lyt-2.1+ and asialo GM1- cells, and the coexistence of unstimulated accessory cells was also essential for the LHF production. Cells responding to both LHF and rIL 2 to generate LAK activity were Thy-1.2-, Lyt-1.1-, Lyt-2.1- and asialo GM1+. The culture fluid of spleen cells stimulated with both N-CWS and rIL 2 contained no tumor necrosis factor (TNF) activity, and the additional stimulation with N-CWS caused no production of either IL 2 or interferon (IFN). Murine recombinant interleukin 1 alpha (Mu-rIL 1 alpha) could not replace the augmentative effect of N-CWS on LAK cell induction. These results suggest that in the presence of rIL 2, N-CWS stimulates murine T cells to produce LHF that is probably distinct from IL 1, IL 2, TNF and IFN.
在用次优剂量的人重组白细胞介素2（rIL-2）进行4天培养之前，将C3H/HeN小鼠脾细胞暴露于红色诺卡氏菌细胞壁骨架（N-CWS）4小时，可增强淋巴因子激活的杀伤（LAK）细胞活性的诱导，而单独用N-CWS处理则不诱导细胞毒性。与此一致的是，N-CWS和rIL-2联合刺激可增强脾细胞的IL-2结合活性。增强的细胞毒性由Thy-1.2 +、Lyt-1.1 -、Lyt-2.1 -和无唾液酸GM1 +细胞介导。扩散小室中的细胞培养显示，用rIL-2培养时，经N-CWS处理的脾细胞产生了一种LAK细胞诱导辅助因子（LAK辅助因子，LHF）。LHF的产生需要Thy-1.2 +、Lyt-1.1 +、Lyt-2.1 +和无唾液酸GM1 -细胞，未刺激的辅助细胞的共存对LHF的产生也至关重要。对LHF和rIL-2均有反应以产生LAK活性的细胞是Thy-1.2 -、Lyt-1.1 -、Lyt-2.1 -和无唾液酸GM1 +。用N-CWS和rIL-2刺激的脾细胞培养液中不含肿瘤坏死因子（TNF）活性，用N-CWS额外刺激不会导致IL-2或干扰素（IFN）的产生。小鼠重组白细胞介素1α（Mu-rIL-1α）不能替代N-CWS对LAK细胞诱导的增强作用。这些结果表明，在rIL-2存在的情况下，N-CWS刺激小鼠T细胞产生可能不同于IL-1、IL-2、TNF和IFN的LHF。
