铜绿假单胞菌的膜蛋白质组学

Membrane proteomics of Pseudomonas aeruginosa.

作者信息

Magnowska Zofia, Hartmann Isabel, Jänsch Lothar, Jahn Dieter

机构信息

Microbial Proteomics, Helmholtz Centre for Infection Research (HZI), Inhoffenstr, Braunschweig, Germany.

出版信息

Methods Mol Biol. 2014;1149:213-24. doi: 10.1007/978-1-4939-0473-0_18.

DOI:10.1007/978-1-4939-0473-0_18

PMID:24818908

Abstract

In recent years gel-free proteomics approaches have been increasingly used for global quantitative proteome analyses of multiple prokaryotic organisms, including Pseudomonas aeruginosa. A major advantage of this method is its suitability for the investigation of membrane proteomes. In this chapter, we present a protocol for preparation of proteins from the inner and outer membrane of P. aeruginosa PAO1 grown as a biofilm culture. Parameters for quantitative protein measurements by 2D-LC-MS/MS are described.

摘要

近年来，无凝胶蛋白质组学方法越来越多地用于多种原核生物（包括铜绿假单胞菌）的全球定量蛋白质组分析。该方法的一个主要优点是其适用于膜蛋白质组的研究。在本章中，我们介绍了一种从作为生物膜培养物生长的铜绿假单胞菌PAO1的内膜和外膜制备蛋白质的方案。描述了通过二维液相色谱-串联质谱进行定量蛋白质测量的参数。