重组大肠杆菌DH5α产植酸酶的培养条件
Cultivation Conditions for Phytase Production from Recombinant Escherichia coli DH5α.
作者信息
Ariff Rafidah Mohd, Fitrianto Anwar, Abd Manap Mohd Yazid, Ideris Aini, Kassim Azhar, Suhairin Afinah, Hussin Anis Shobirin Meor
机构信息
Department of Food Technology, Faculty of Food Science and Technology, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
Department of Mathematics, Faculty of Science, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
出版信息
Microbiol Insights. 2013 Apr 9;6:17-28. doi: 10.4137/MBI.S10402. eCollection 2013.
Response surface methodology (RSM) was used to optimize the cultivation conditions for the production of phytase by recombinant Escherichia coli DH5α. The optimum predicted cultivation conditions for phytase production were at 3 hours seed age, a 2.5% inoculum level, an L-arabinose concentration of 0.20%, a cell concentration of 0.3 (as measured at 600 nm) and 17 hours post-induction time with a predicted phytase activity of 4194.45 U/mL. The model was validated and the results showed no significant difference between the experimental and the predicted phytase activity (P = 0.305). Under optimum cultivation conditions, the phytase activity of the recombinant E. coli DH5α was 364 times higher compared to the phytase activity of the wild-type producer, Enterobacter sakazakii ASUIA279. Hence, optimization of the cultivation conditions using RSM positively increased phytase production from recombinant E. coli DH5α.
采用响应面法（RSM）优化重组大肠杆菌DH5α生产植酸酶的培养条件。预测的植酸酶生产最佳培养条件为种子培养3小时、接种量2.5%、L-阿拉伯糖浓度0.20%、细胞浓度0.3（在600nm处测量）以及诱导后17小时，预测的植酸酶活性为4194.45U/mL。对该模型进行了验证，结果表明实验测得的植酸酶活性与预测值之间无显著差异（P = 0.305）。在最佳培养条件下，重组大肠杆菌DH5α的植酸酶活性比野生型产酶菌阪崎肠杆菌ASUIA279的植酸酶活性高364倍。因此，使用响应面法优化培养条件可有效提高重组大肠杆菌DH5α的植酸酶产量。
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