[2011年北京儿童A群链球菌超抗原分布研究]

[Study on the distribution of superantigen of group A streptococcus isolated from children in Beijing, 2011].

作者信息

Peng Xiaomin, Liu Shuang, Yang Peng, Li Jing, Zhang Daitao, Cui Shujuan, Wu Shuangsheng, Liu Yimeng, Wang Quanyi

机构信息

Institute for Infectious Disease and Endemic Disease Control, Beijing Centers for Disease Control and Prevention, Beijing 100013, China.

Institute for Infectious Disease and Endemic Disease Control, Beijing Centers for Disease Control and Prevention, Beijing 100013, China. Email:

出版信息

Zhonghua Liu Xing Bing Xue Za Zhi. 2014 Mar;35(3):299-302.

PMID:24831631

Abstract

OBJECTIVE

To analyze the prevalence of super-antigens (SAgs) of group A streptococcus (GAS)isolated from Beijing pediatric patients in 2011, and to explore the relationship between emm types, characteristics of patients and SAgs.

METHODS

A total of 635 isolates of GAS were collected from children in 36 hospitals in Beijing from May to July, 2011. Thirteen currently known SAg genes were tested by real-time PCR, and emm gene was performed by PCR and sequencing of N-terminal gene fragments of M protein.

RESULTS

Prevalence rates of 13 SAg genes speA, speB, speC, speF, speG, speH, speI, speJ, speK, speL, speM, smeZ, ssa were 22.4%, 100.0%, 99.4%, 99.7%, 99.7%, 76.4%, 76.2%, 21.7%,0.6%, 1.1%, 2.2%, 99.7% and 98.0%, respectively. A total of 26 SAgs profiles were observed according to the SAgs inclusion. There were significant differences in frequencies of speA, speH, speI and speJ between emm1 and emm12 strains (P < 0.05). In isolates from patients with pharyngeal infection, the prevalence rates for speK and speL were higher while the frequency for ssa was lower than that from scarlet fever cases (P < 0.05).

CONCLUSION

The frequencies of speB, speF, smeZ, speG, speC, and ssa were high among strains isolated while speM, speL and speK were relatively low from children in Beijing, 2011. SAg genes appeared to be associated with the emm types. In this study, differences of frequency for speA and speC from strains collected from patients with scarlet fever and pharyngeal infection had not been found.

摘要

目的

分析2011年从北京儿科患者中分离出的A组链球菌（GAS）超抗原（SAgs）的流行情况，并探讨emm型、患者特征与SAgs之间的关系。

方法

2011年5月至7月，从北京36家医院的儿童中收集了635株GAS分离株。通过实时PCR检测13个目前已知的SAg基因，并通过PCR和M蛋白N端基因片段测序进行emm基因检测。

结果

13个SAg基因speA、speB、speC、speF、speG、speH、speI、speJ、speK、speL、speM、smeZ、ssa的流行率分别为22.4%、100.0%、99.4%、99.7%、99.7%、76.4%、76.2%、21.7%、0.6%、1.1%、2.2%、99.7%和98.0%。根据SAgs包含情况共观察到26种SAgs谱型。emm1和emm12菌株之间speA、speH、speI和speJ的频率存在显著差异（P<0.05）。在咽感染患者的分离株中，speK和speL的流行率较高，而ssa的频率低于猩红热病例（P<0.05）。