Zybina T G, Zybina E V
Tsitologiia. 1989 Nov;31(11):1292-305.
A cytomorphological study was made of silver stained nucleoli in interphasic nuclei of trophoblast cells from the rat placenta connective zone, in addition to calculation of Ag-positive spherules in the nucleoli. The prevalent number of Ag-positive nucleolar spherules in the nuclei was 6, corresponding to the number of nucleolar organizers (NOR's) in the diploid chromosome complement of the rat. The mean number of Ag-positive spherules in the nucleoli progressively increase in the course of polyploidization from 2c to 32c; variability of the spherule number also increasing. The mean area of nucleoli is found to increase in proportion to the ploidy degree. A high correlation is found between the number of Ag-positive spherules and the area of nucleoli in the nucleus (r = 0.78). This appropriateness is exhibited at all the ploidy levels. The number of Ag-spherules and the area of nucleoli are found to depend slightly on the number of nucleoli. The possibility to use the number of Ag-positive spherules as a criterion of the activity of the NOR in interphasic nuclei is discussed.
除了计算核仁中银阳性小球的数量外，还对大鼠胎盘连接区滋养层细胞间期核中经银染色的核仁进行了细胞形态学研究。核中银阳性核仁小球的普遍数量为6，与大鼠二倍体染色体组中的核仁组织者（NOR）数量相对应。在从2c到32c的多倍体化过程中，核仁中银阳性小球的平均数量逐渐增加；小球数量的变异性也增加。发现核仁的平均面积与倍性程度成比例增加。核中银阳性小球的数量与核仁面积之间存在高度相关性（r = 0.78）。这种相关性在所有倍性水平上均表现出来。发现银小球的数量和核仁面积略微依赖于核仁的数量。讨论了将银阳性小球的数量用作间期核中NOR活性标准的可能性。
