Yamada Takanori, Wei Min, Toyoda Takeshi, Yamano Shoutaro, Wanibuchi Hideki
Department of Pathology, Osaka City University Graduate School of Medicine, Osaka 545-8585, Japan.
Division of Pathology, National Institute of Health Sciences, Tokyo 158-8501, Japan.
Food Chem Toxicol. 2014 Aug;70:107-13. doi: 10.1016/j.fct.2014.04.037. Epub 2014 May 13.
Helicobacter pylori (H. pylori) infection is well known to be associated with chronic gastritis and also development of gastric cancer. Raphanobrassica (RB) is an intergeneric hybrid of the genera Raphanus (radish) and Brassica (cabbages) containing appreciable amounts of glucoraphanin (GR) and glucoraphenin (GRe), which are actively hydrolyzed by the enzyme myrosinase to sulforaphane and sulforaphene, respectively. Both of these metabolites exert antimicrobial and anti-inflammatory activity. The purpose of the present study was to investigate the effect of two freeze-dried products of RB (RB1 and RB2) on H. pylori-induced gastritis in Mongolian gerbils. Six-week-old male Mongolian gerbils were inoculated orally with H. pylori (ATCC 43504), and 2weeks later were fed diets containing no additives or diets supplemented with 2% RB1 (containing both GR and GRe) or 2% RB2 (containing GR only) for 10weeks. In the RB1, but not the RB2 group, mononuclear cell infiltration, mRNA expression of IL-6, and cell proliferation in the gastric mucosa were significantly suppressed. These results indicate that RB1 containing both GR and GRe exerted significant inhibitory effects on H. pylori-induced gastritis in Mongolian gerbils apparently mediated via suppression of IL-6 expression and chronic inflammation.
众所周知，幽门螺杆菌（H. pylori）感染与慢性胃炎以及胃癌的发生有关。萝卜甘蓝（RB）是萝卜属（萝卜）和芸苔属（卷心菜）的属间杂种，含有大量的萝卜硫苷（GR）和萝卜硫素（GRe），它们分别被黑芥子酶积极水解为萝卜硫素和萝卜硫烯。这两种代谢产物都具有抗菌和抗炎活性。本研究的目的是调查RB的两种冻干产品（RB1和RB2）对蒙古沙鼠幽门螺杆菌诱导的胃炎的影响。六周龄雄性蒙古沙鼠经口接种幽门螺杆菌（ATCC 43504），两周后喂食不含添加剂的饮食或补充2% RB1（同时含有GR和GRe）或2% RB2（仅含有GR）的饮食10周。在RB1组而非RB2组中，胃黏膜中的单核细胞浸润、IL-6的mRNA表达和细胞增殖均受到显著抑制。这些结果表明，同时含有GR和GRe的RB1对蒙古沙鼠幽门螺杆菌诱导的胃炎具有显著抑制作用，这显然是通过抑制IL-6表达和慢性炎症介导的。
