• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

原发性醛固酮增多症中肾上腺CYP11B1/2的表达：使用新型单克隆抗体的免疫组织化学分析

Adrenal CYP11B1/2 expression in primary aldosteronism: immunohistochemical analysis using novel monoclonal antibodies.

作者信息

Nakamura Yasuhiro, Maekawa Takashi, Felizola Saulo J A, Satoh Fumitoshi, Qi Xin, Velarde-Miranda Carolina, Plonczynski Maria W, Ise Kazue, Kikuchi Kumi, Rainey William E, Gomez-Sanchez Elise P, Gomez-Sanchez Celso E, Sasano Hironobu

机构信息

Tohoku University Graduate School of Medicine, Department of Pathology, Sendai, Japan.

Tohoku University Hospital, Division of Nephrology and Hypertension, Sendai, Japan.

出版信息

Mol Cell Endocrinol. 2014 Jul 5;392(1-2):73-9. doi: 10.1016/j.mce.2014.05.002. Epub 2014 May 14.

DOI:10.1016/j.mce.2014.05.002
PMID:24837548
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5471353/
Abstract

CYP11B1 and CYP11B2 play pivotal roles in adrenocorticosteroids synthesis. We performed semi-quantitative immunohistochemical analysis of these proteins in adrenals from patients with primary aldosteronism using novel monoclonal antibodies. Clusters of cortical cells positive for CYP11B2 were detected in the zona glomerulosa (ZG) of normal adrenal gland (NA), idiopathic hyperaldosteronism (IHA) and the adjacent adrenal of aldosterone-producing adenoma (APA). In APA, heterogenous immunolocalization of CYP11B2 and diffuse immunoreactivity of CYP11B1 were detected in tumor cells, respectively. The relative immunoreactivity of CYP11B2 in the ZG of adjacent adrenal of APA was significantly lower than that of NA, IHA and APA tumor cells, suggestive of suppressed aldosterone biosynthesis in these cells. These findings did indicate the regulatory mechanisms of aldosterone biosynthesis were different between normal/hyperplastic and neoplastic aldosterone-producing cells in human adrenals. CYP11B2 immunoreactivity in the ZG could also serve as a potential immunohistochemical marker differentiating morphologically hyperplastic ZG of IHA and APA adjacent adrenal.

摘要

细胞色素P450 11B1（CYP11B1）和细胞色素P450 11B2（CYP11B2）在肾上腺皮质类固醇合成中起关键作用。我们使用新型单克隆抗体对原发性醛固酮增多症患者肾上腺中的这些蛋白质进行了半定量免疫组织化学分析。在正常肾上腺（NA）、特发性醛固酮增多症（IHA）以及醛固酮瘤（APA）的相邻肾上腺的球状带（ZG）中检测到CYP11B2阳性的皮质细胞簇。在APA中，分别在肿瘤细胞中检测到CYP11B2的异质性免疫定位和CYP11B1的弥漫性免疫反应性。APA相邻肾上腺ZG中CYP11B2的相对免疫反应性显著低于NA、IHA和APA肿瘤细胞，提示这些细胞中醛固酮生物合成受到抑制。这些发现确实表明，人肾上腺中正常/增生性和肿瘤性醛固酮产生细胞之间醛固酮生物合成的调节机制不同。ZG中CYP11B2免疫反应性也可作为区分IHA和APA相邻肾上腺形态学增生性ZG的潜在免疫组织化学标志物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d3d6/5471353/154008d2c3ac/nihms867607f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d3d6/5471353/6be50cb68ec1/nihms867607f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d3d6/5471353/bbbf3a22e51d/nihms867607f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d3d6/5471353/0d94a6aeeb42/nihms867607f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d3d6/5471353/154008d2c3ac/nihms867607f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d3d6/5471353/6be50cb68ec1/nihms867607f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d3d6/5471353/bbbf3a22e51d/nihms867607f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d3d6/5471353/0d94a6aeeb42/nihms867607f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d3d6/5471353/154008d2c3ac/nihms867607f4.jpg

相似文献

1
Adrenal CYP11B1/2 expression in primary aldosteronism: immunohistochemical analysis using novel monoclonal antibodies.原发性醛固酮增多症中肾上腺CYP11B1/2的表达：使用新型单克隆抗体的免疫组织化学分析
Mol Cell Endocrinol. 2014 Jul 5;392(1-2):73-9. doi: 10.1016/j.mce.2014.05.002. Epub 2014 May 14.
2
Adrenal cortex remodeling and functional zona glomerulosa hyperplasia in primary aldosteronism.原发性醛固酮增多症中的肾上腺皮质重塑和功能球状带增生。
Hypertension. 2010 Nov;56(5):885-92. doi: 10.1161/HYPERTENSIONAHA.110.158543. Epub 2010 Oct 11.
3
Quantitative assessment of CYP11B1 and CYP11B2 expression in aldosterone-producing adenomas.醛固酮分泌性腺瘤中CYP11B1和CYP11B2表达的定量评估。
Eur J Endocrinol. 2002 Dec;147(6):795-802. doi: 10.1530/eje.0.1470795.
4
Immunohistochemical, genetic and clinical characterization of sporadic aldosterone-producing adenomas.散发性醛固酮瘤的免疫组织化学、遗传学及临床特征
Mol Cell Endocrinol. 2015 Aug 15;411:146-54. doi: 10.1016/j.mce.2015.04.022. Epub 2015 May 6.
5
Immunohistochemistry of aldosterone synthase leads the way to the pathogenesis of primary aldosteronism.醛固酮合酶的免疫组织化学为原发性醛固酮增多症的发病机制研究指明了方向。
Mol Cell Endocrinol. 2017 Feb 5;441:124-133. doi: 10.1016/j.mce.2016.10.014. Epub 2016 Oct 14.
6
PCP4: a regulator of aldosterone synthesis in human adrenocortical tissues.PCP4：人肾上腺皮质组织中醛固酮合成的调节剂。
J Mol Endocrinol. 2014 Feb 24;52(2):159-67. doi: 10.1530/JME-13-0248. Print 2014 Apr.
7
Disordered zonal and cellular CYP11B2 enzyme expression in familial hyperaldosteronism type 3.3型家族性醛固酮增多症中肾上腺带区和细胞的CYP11B2酶表达紊乱。
Mol Cell Endocrinol. 2017 Jan 5;439:74-80. doi: 10.1016/j.mce.2016.10.025. Epub 2016 Oct 25.
8
Genetic, Cellular, and Molecular Heterogeneity in Adrenals With Aldosterone-Producing Adenoma.醛固酮瘤肾上腺组织中的遗传、细胞和分子异质性。
Hypertension. 2020 Apr;75(4):1034-1044. doi: 10.1161/HYPERTENSIONAHA.119.14177. Epub 2020 Mar 2.
9
Histopathological and genetic characterization of aldosterone-producing adenomas with concurrent subclinical cortisol hypersecretion: a case series.伴有亚临床皮质醇过度分泌的醛固酮瘤的组织病理学和遗传学特征：病例系列研究。
Endocrine. 2017 Dec;58(3):503-512. doi: 10.1007/s12020-017-1295-4. Epub 2017 Apr 12.
10
Isoform-specific monoclonal antibodies against 3β-hydroxysteroid dehydrogenase/isomerase family provide markers for subclassification of human primary aldosteronism.针对3β-羟基类固醇脱氢酶/异构酶家族的亚型特异性单克隆抗体为人类原发性醛固酮增多症的亚分类提供了标志物。
J Clin Endocrinol Metab. 2014 Feb;99(2):E257-62. doi: 10.1210/jc.2013-3279. Epub 2013 Jan 1.

引用本文的文献

1
Lipid Metabolism Reprogramming in Cancer: Insights into Tumor Cells and Immune Cells Within the Tumor Microenvironment.癌症中的脂质代谢重编程：对肿瘤微环境中肿瘤细胞和免疫细胞的见解
Biomedicines. 2025 Aug 4;13(8):1895. doi: 10.3390/biomedicines13081895.
2
Intratumoural aldosterone and CYP11B2 expression levels among different genotypes of aldosterone producing tumours.醛固酮分泌瘤不同基因型中的肿瘤内醛固酮和CYP11B2表达水平。
Endocr Connect. 2025 Jun 26;14(6). doi: 10.1530/EC-25-0070. Print 2025 Jun 1.
3
EGR1 regulates oxidative stress and aldosterone production in adrenal cells and aldosterone-producing adenomas.

本文引用的文献

1
PCP4: a regulator of aldosterone synthesis in human adrenocortical tissues.PCP4：人肾上腺皮质组织中醛固酮合成的调节剂。
J Mol Endocrinol. 2014 Feb 24;52(2):159-67. doi: 10.1530/JME-13-0248. Print 2014 Apr.
2
Development of monoclonal antibodies against human CYP11B1 and CYP11B2.针对人 CYP11B1 和 CYP11B2 的单克隆抗体的开发。
Mol Cell Endocrinol. 2014 Mar 5;383(1-2):111-7. doi: 10.1016/j.mce.2013.11.022. Epub 2013 Dec 8.
3
Glutamate receptors and the regulation of steroidogenesis in the human adrenal gland: the metabotropic pathway.
EGR1调节肾上腺细胞和醛固酮瘤中的氧化应激及醛固酮生成。
Redox Biol. 2025 Mar;80:103498. doi: 10.1016/j.redox.2025.103498. Epub 2025 Jan 15.
4
Evaluating the role of aldosterone synthesis on adrenal cell fate.评估醛固酮合成对肾上腺细胞命运的作用。
Front Endocrinol (Lausanne). 2024 Aug 7;15:1423027. doi: 10.3389/fendo.2024.1423027. eCollection 2024.
5
Evaluation of adrenal vein anatomy by adrenal venous sampling in patients with primary aldosteronism in Chinese.中国人原发性醛固酮增多症患者肾上腺静脉采血的肾上腺静脉解剖评估。
J Clin Hypertens (Greenwich). 2024 Aug;26(8):912-920. doi: 10.1111/jch.14860. Epub 2024 Jun 27.
6
Double CYP11B1/CYP11B2 Immunohistochemistry and Detection of KCNJ5 Mutations in Primary Aldosteronism.原发性醛固酮增多症中 CYP11B1/CYP11B2 双重免疫组化和 KCNJ5 突变检测。
J Clin Endocrinol Metab. 2024 Sep 16;109(10):2433-2443. doi: 10.1210/clinem/dgae411.
7
Unilateral Primary Aldosteronism: Long-Term Disease Recurrence After Adrenalectomy.单侧醛固酮增多症：肾上腺切除术后的长期疾病复发。
Hypertension. 2024 Apr;81(4):936-945. doi: 10.1161/HYPERTENSIONAHA.123.22281. Epub 2024 Feb 6.
8
Advances in the molecular imaging of primary aldosteronism.原发性醛固酮增多症的分子影像学进展。
Ann Nucl Med. 2023 Aug;37(8):433-441. doi: 10.1007/s12149-023-01851-y. Epub 2023 Jul 2.
9
The Driver Role of Pathologists in Endocrine Oncology: What Clinicians Seek in Pathology Reports.病理学家在内分泌肿瘤学中的角色：临床医生在病理报告中寻求什么。
Endocr Pathol. 2023 Dec;34(4):437-454. doi: 10.1007/s12022-023-09768-y. Epub 2023 May 11.
10
Exploring the potential of late permian aggregate resources for utilization in engineering structures through geotechnical, geochemical and petrographic analyses.通过岩土工程、地球化学和岩相学分析，探索晚二叠世聚合资源在工程结构中的利用潜力。
Sci Rep. 2023 Mar 29;13(1):5088. doi: 10.1038/s41598-023-32294-0.
谷氨酸受体与人类肾上腺甾体生成的调节：代谢型途径。
Mol Cell Endocrinol. 2014 Jan 25;382(1):170-177. doi: 10.1016/j.mce.2013.09.025. Epub 2013 Sep 27.
4
Histopathological diagnosis of primary aldosteronism using CYP11B2 immunohistochemistry.使用 CYP11B2 免疫组织化学对原发性醛固酮增多症进行组织病理学诊断。
J Clin Endocrinol Metab. 2013 Apr;98(4):1567-74. doi: 10.1210/jc.2012-3726. Epub 2013 Feb 26.
5
Characterization of steroidogenic enzyme expression in aldosterone-producing adenoma: a comparison with various human adrenal tumors.醛固酮瘤中甾体激素生成酶表达的特征：与各种人肾上腺肿瘤的比较。
Endocr J. 2013;60(3):329-36. doi: 10.1507/endocrj.ej12-0270. Epub 2012 Dec 18.
6
Microarray, qPCR, and KCNJ5 sequencing of aldosterone-producing adenomas reveal differences in genotype and phenotype between zona glomerulosa- and zona fasciculata-like tumors.对醛固酮瘤进行微阵列、qPCR 和 KCNJ5 测序，揭示了球状带样和束状带样肿瘤在基因型和表型上的差异。
J Clin Endocrinol Metab. 2012 May;97(5):E819-29. doi: 10.1210/jc.2011-2965. Epub 2012 Mar 22.
7
Gene expression profiles in aldosterone-producing adenomas and adjacent adrenal glands.醛固酮腺瘤及毗邻肾上腺组织中的基因表达谱。
Eur J Endocrinol. 2011 Apr;164(4):613-9. doi: 10.1530/EJE-10-1085. Epub 2011 Jan 19.
8
Adrenocortical zonation in humans under normal and pathological conditions.人类在正常和病理条件下的肾上腺皮质分区。
J Clin Endocrinol Metab. 2010 May;95(5):2296-305. doi: 10.1210/jc.2009-2010. Epub 2010 Mar 3.
9
Case detection, diagnosis, and treatment of patients with primary aldosteronism: an endocrine society clinical practice guideline.原发性醛固酮增多症患者的病例发现、诊断及治疗：内分泌学会临床实践指南
J Clin Endocrinol Metab. 2008 Sep;93(9):3266-81. doi: 10.1210/jc.2008-0104. Epub 2008 Jun 13.
10
Primary aldosteronism: renaissance of a syndrome.原发性醛固酮增多症：一种综合征的复兴
Clin Endocrinol (Oxf). 2007 May;66(5):607-18. doi: 10.1111/j.1365-2265.2007.02775.x.