Stein-Streilein J, Guffee J, Ramos M, Pitchenik A E
Department of Medicine, University of Miami School of Medicine, FL 33101.
Reg Immunol. 1989 Nov-Dec;2(6):370-5.
Bronchoalveolar lavage cells (BAC) are considered to be representative of cells that are in the interstitium and in patients with lung cancer, may represent, in part, cells that infiltrate cancerous tissue. We used bronchoalveolar lavage (BAL) specimens to test the hypothesis that cells within this region might be regulated locally by factors and show activities that are dependent on these growth and activation mediators. We showed previously that Natural Killer (NK) activity and IL2 titers were proportional. As compared to normal subjects, patients with all stages of bronchogenic carcinoma consistently had very high levels of IL2 in their bronchoalveolar lavage (BAL) fluid and this titer correlated with an increase in NK activity in the BAC both in absolute level and in relative level to the blood. Now, we report results that show that spontaneous lymphokine activated killer (LAK) activity also can be measured in most patients, but not all patients, that express IL2 titers. These findings support the hypotheses that different types of nonspecific cytotoxic cells are present and active in cancerous lung specimens secondary to the secretion of lymphokines from activated T lymphocytes in that region and that LAK activity is a physiological phenomenon that may be expressed in regional rather than systemic areas of the body.
支气管肺泡灌洗细胞（BAC）被认为是间质中细胞的代表，在肺癌患者中，可能部分代表浸润癌组织的细胞。我们使用支气管肺泡灌洗（BAL）标本检验这样一个假设：该区域内的细胞可能受局部因子调控，并表现出依赖于这些生长和激活介质的活性。我们之前表明自然杀伤（NK）活性与白细胞介素2（IL2）滴度成比例。与正常受试者相比，各期支气管源性癌患者的支气管肺泡灌洗（BAL）液中IL2水平始终非常高，且该滴度与BAC中NK活性的增加在绝对水平以及相对于血液的相对水平上均相关。现在，我们报告的结果表明，在大多数（但并非所有）表达IL2滴度的患者中也可检测到自发的淋巴因子激活的杀伤细胞（LAK）活性。这些发现支持以下假设：在该区域激活的T淋巴细胞分泌淋巴因子后，不同类型的非特异性细胞毒性细胞存在于癌性肺标本中并具有活性，且LAK活性是一种可能在身体局部而非全身区域表现出来的生理现象。
