[微小RNA相关基因（DROSHA、DICER1和GEMIN4）多态性与T细胞淋巴瘤预后的关联]
[Association of microRNA-related genes (DROSHA, DICER1 and GEMIN4) polymorphisms with T-cell lymphoma prognosis].
作者信息
Tian Xiaobo, Zhang Bo, Li Xi, Zhai Kan, Xu Jian, Chang Jiang, Qiao Yan, Zhou Yuling, Huang Liming, Chen Jieping
机构信息
Department of Hematology, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
出版信息
Zhonghua Xue Ye Xue Za Zhi. 2014 May;35(5):408-13. doi: 10.3760/cma.j.issn.0253-2727.2014.05.007.
OBJECTIVE
To analyze the association of micoRNA-related genes DROSHA single nucleotide polymorphisms (SNP) rs10719 and rs6877842, DICER1 rs3742330and GEMIN4 rs3744741 with prognosis of T-cell lymphoma.
METHODS
Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was used to determine the genotypes of the above 4SNPs and their associations with complete remission (CR) rate and overall survival (OS) in 163 patients with TCL.
RESULTS
Patients carrying the rs6877842 CG genotype had a significantly higher CR rate compared with those carrying the CC genotype (OR=0.07, 95% CI 0.01-0.72, P=0.026); the same for patients carrying the DICER1 rs3742330 GG genotype compared with those carrying the GA genotype (OR=0.15, 95% CI 0.02-0.97, P=0.047) or the AA genotype (OR=0.11, 95% CI 0.02-0.71, P=0.020). In addition, patients with the DICER1 rs3742330 GG genotype had a significantly improved OS compared with those carrying the GA (HR=9.02, 95% CI 1.22-66.92, P=0.031) or AA genotype (HR=8.77, 95% CI 1.19-64.67, P=0.033). The other two SNPs of rs10719 and rs3744741 had no significant association with CR or OS.
CONCLUSION
DROSHA rs6877842 and DICER1 rs3742330 were independent factors for TCL CR, and DICER1 rs3742330 was also an independent prognostic factor for TCL OS.
目的
分析微小RNA相关基因DROSHA单核苷酸多态性（SNP）rs10719和rs6877842、DICER1 rs3742330以及GEMIN4 rs3744741与T细胞淋巴瘤预后的相关性。
方法
采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法，对163例TCL患者上述4个SNP的基因型及其与完全缓解（CR）率和总生存期（OS）的相关性进行检测。
结果
携带rs6877842 CG基因型的患者与携带CC基因型的患者相比，CR率显著更高（OR=0.07，95%CI 0.01-0.72，P=0.026）；携带DICER1 rs3742330 GG基因型的患者与携带GA基因型（OR=0.15，95%CI 0.02-0.97，P=0.047）或AA基因型（OR=0.11，95%CI 0.02-0.71，P=0.020）的患者相比，情况相同。此外，携带DICER1 rs3742330 GG基因型的患者与携带GA基因型（HR=9.02，95%CI 1.22-66.92，P=0.031）或AA基因型（HR=8.77，95%CI 1.19-64.67，P=0.033）的患者相比，OS显著改善。另外两个SNP，即rs10719和rs3744741，与CR或OS无显著相关性。
结论
DROSHA rs6877842和DICER1 rs3742330是TCL患者CR的独立影响因素，DICER1 rs3742330也是TCL患者OS的独立预后因素。
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