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超声辐射与离子液体预处理相结合用于稻草的酶解

Combination of ultrasonic irradiation with ionic liquid pretreatment for enzymatic hydrolysis of rice straw.

机构信息

Department of Food Science and Biotechnology, National Chung Hsing University, 250 Kuokuang Road, Taichung 40227, Taiwan, ROC.

Department of Food Science and Biotechnology, National Chung Hsing University, 250 Kuokuang Road, Taichung 40227, Taiwan, ROC; Department of Nutrition, China Medical University, 91 Hsueh Shih Road, Taichung 40402, Taiwan, ROC.

出版信息

Bioresour Technol. 2014 Jul;164:198-202. doi: 10.1016/j.biortech.2014.05.004. Epub 2014 May 10.

Abstract

The application of ultrasonic irradiation and ionic liquids (ILs) in the degradation of rice straw under different processes of pretreatment and enzymatic hydrolysis was investigated. Various substrates for enzymatic hydrolysis by cellulase with and without ultrasound were as follows: untreated rice-straw powder (RS); RS treated by ILs of 1-ethyl-3-methylimidazolium ethylsulfate and trihexyl (tetradecyl) phosphonium decanoate with ultrasound at 300 W/(40 kHz, 28 kHz); RS treated by IL of choline hydroxide ([Ch][OH]) with ultrasound at 300 W/40 kHz (CHRS). In ultrasound-mediated enzymatic hydrolysis, the yield of total reducing sugar (TRS) converted from CHRS was up to 96.22% at 240 min and was greater than that from the other substrates; the TRS yield for CHRS with ultrasound was 19.5% greater than that without irradiation. Lignocellulosic biomass pretreated with [Ch][OH] showed the highest efficiency among the tested ILs, and ultrasound can be applied effectively in rice-straw pretreatment and enzymatic hydrolysis.

摘要

研究了超声辐射和离子液体(ILs)在不同预处理和酶解过程中对稻草降解的应用。纤维素酶酶解的各种底物如下:未经处理的稻草粉(RS);经 1-乙基-3-甲基咪唑硫酸乙酯和三己基(十四烷基)磷酸正癸酯的 IL 超声处理(300 W/(40 kHz,28 kHz));经胆碱氢氧化 [Ch][OH] 和超声处理(300 W/40 kHz,CHRS)的 IL。在超声介导的酶解中,CHRS 转化的总还原糖(TRS)产率在 240 分钟时高达 96.22%,高于其他底物;超声处理的 CHRS 的 TRS 产率比没有照射时高 19.5%。在测试的 ILs 中,预处理用 [Ch][OH] 的木质纤维素生物质表现出最高的效率,超声可有效应用于稻草预处理和酶解。

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