Synthesis of buffers for generating immobilized pH gradients. I: Acidic acrylamido buffers.
作者信息
Chiari M, Casale E, Santaniello E, Righetti P G
机构信息
Department of Biomedical Sciences and Technologies, University of Milano, Italy.
出版信息
Appl Theor Electrophor. 1989;1(2):99-102.
PMID:2488594
Abstract
Five acidic acrylamido buffers for isoelectric focusing in immobilized pH gradients, are described: a strong titrant (pK 1.0) 2-acrylamido-2-methyl propane sulphonic acid and four weaker acids, namely 2-acrylamido glycolic acid (pK 3.1), N-acryloil glycine (pK 3.6), 3-acrylamido propanoic acid (pK 4.4) and 4-acrylamido butyric acid (pK 4.6). Their synthesis and general properties are described.
摘要
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