Akoglu Bora, Lafferton Barbara, Kalb Shara, Yosuf Said Emal, Herrmann Eva, Zeuzem Stefan, Goßmann Jan, Kachel Heinz-Georg, Scheuermann Ernst-H, Faust Dominik
Asklepios Klinik Langen, Medizinische Klinik 2, Röntgenstrasse 20, Langen, Germany.
Klinikum der Johann Wolfgang Goethe-Universität, Medizinische Klinik 1, Theodor-Stern-Kai 7, Frankfurt am Main, Germany.
Transpl Immunol. 2014 Jun;31(1):17-21. doi: 10.1016/j.trim.2014.05.004. Epub 2014 Jun 2.
CD8+ T-cells and interleukin-2 play an important role during organ rejection in kidney transplant recipients. Numerous studies showed increased interleukin-2 levels during acute rejection. The aim of our study is to show an association between intracellular interleukin-2 in CD8+ T-cells and the incidence of those who underwent organ rejection in kidney transplant recipients.
407 transplant recipients were included into this study. The rejection incidence was investigated from the patient records. White blood cells from recipients were separated using a ficoll gradient. The cells were double-gated (CD3+ and CD8+) for the analysis of cellular percentage for intracellular interleukin-2 with a flow cytometer.
The percentage of CD8+ cells with detectable intracellular interleukin-2 was significantly higher in renal transplant recipients with a documented rejection compared to recipients without any history of rejection (9.06±0.50, N=133 vs. 4.28±0.24, N=274, p<0.0001, t-test). Further, there was a significant increase in patients with one (8.02±0.54, N=80, p<0.0001, t-test), two (10.40±1.17, N=33, p<0.0001, t-test) or three (11.82±1.58, N=18, p<0.0001, t-test) rejection events.
Past studies showed, that during acute organ rejection intracellular interleukin-2 is increased in cytotoxic T-cells, supposed to be a marker for this event. We were able to show, that intracellular interleukin-2 in CD8+ T-cells is also increased after organ rejection. Furthermore it seems to depend on the quantity of rejection events. Further studies in recipients with increased intracellular interleukin-2 in cytotoxic CD8+ T-cells and documented history of organ rejection are needed to identify this as a possible risk factor for further rejection events.
CD8 + T细胞和白细胞介素-2在肾移植受者的器官排斥反应中起重要作用。大量研究表明,急性排斥反应期间白细胞介素-2水平会升高。我们研究的目的是表明肾移植受者CD8 + T细胞内白细胞介素-2与发生器官排斥反应者的发生率之间存在关联。
407名移植受者纳入本研究。通过患者记录调查排斥反应发生率。使用菲可梯度分离受者的白细胞。用流式细胞仪对细胞进行双门控(CD3 +和CD8 +),以分析细胞内白细胞介素-2的细胞百分比。
有记录的排斥反应的肾移植受者中,可检测到细胞内白细胞介素-2的CD8 +细胞百分比显著高于无任何排斥反应史的受者(9.06±0.50,N = 133 vs. 4.28±0.24,N = 274,p <0.0001,t检验)。此外,发生一次(8.02±0.54,N = 8)、两次(10.40±1.17,N = 33,p <0.0001,t检验)或三次(11.82±1.58,N = 18,p <0.0001,t检验)排斥反应事件的患者中,该百分比也显著增加。
既往研究表明,在急性器官排斥反应期间,细胞毒性T细胞内的白细胞介素-2会增加,这被认为是该事件的一个标志物。我们能够证明,器官排斥反应后CD8 + T细胞内的白细胞介素-2也会增加。此外,它似乎取决于排斥反应事件的数量。需要对细胞毒性CD8 + T细胞内白细胞介素-2增加且有器官排斥反应记录史的受者进行进一步研究,以确定这是否为进一步排斥反应事件的可能危险因素。