Cardin A D, Jackson R L, Elledge B, Feldhake D
Merrell Dow Research Institute, Cincinnati, Ohio 45215-6300.
Int J Biol Macromol. 1989 Feb;11(1):59-62. doi: 10.1016/0141-8130(89)90042-1.
Successive rechromatography of commercial bovine lung heparin on human plasma low density lipoproteins (LDL) immobilized to AffiGel-10 yielded four high reactive heparin (HRH-I to IV) fractions and an unreactive fraction (URH). HRH-I was the most sulphated HRH fraction whereas URH had the least sulphation. In the presence of 10 mM Ca2+, LDL were precipitated by these heparins in the following order: HRH-II greater than HRH-III greater than HRH-IV greater than HRH-I greater than URH. The average molecular weight of HRH-I to IV was 8600, 11400, 10,100, and 10,000, respectively. A plot of log molecular weight versus the concentration of HRH required to give half-maximal precipitation of LDL showed a negative correlation (r = -0.880). These results indicate that heparin chain length is an important determinant of heparin binding to LDL in solution and may have relevance to the binding and precipitation of LDL in the arterial wall.
将市售牛肺肝素在固定于AffiGel - 10的人血浆低密度脂蛋白（LDL）上进行连续再色谱分离，得到四个高反应性肝素（HRH - I至IV）组分和一个无反应性组分（URH）。HRH - I是硫酸化程度最高的HRH组分，而URH的硫酸化程度最低。在10 mM Ca2 +存在下，这些肝素使LDL沉淀的顺序如下：HRH - II＞HRH - III＞HRH - IV＞HRH - I＞URH。HRH - I至IV的平均分子量分别为8600、11400、10100和10000。以对数分子量对使LDL达到半数最大沉淀所需的HRH浓度作图，显示出负相关（r = - 0.880）。这些结果表明，肝素链长度是溶液中肝素与LDL结合的重要决定因素，可能与LDL在动脉壁中的结合和沉淀有关。
