Silvani Vanesa Analía, Bidondo Laura Fernández, Bompadre María Josefina, Colombo Roxana Paula, Pérgola Mariana, Bompadre Agustín, Fracchia Sebastián, Godeas Alicia
Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales (FCEyN), Universidad de Buenos Aires (UBA), Ciudad Universitaria, 4to piso, Pabellón 2, 1428, Buenos Aires, Argentina
Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales (FCEyN), Universidad de Buenos Aires (UBA), Ciudad Universitaria, 4to piso, Pabellón 2, 1428, Buenos Aires, Argentina.
Mycologia. 2014 Sep-Oct;106(5):963-75. doi: 10.3852/13-118. Epub 2014 Jun 2.
The growth dynamics of extraradical mycelium and spore formation of 14 "Rhizophagus" isolates from different sites in Argentina were evaluated under monoxenic conditions. A modified Gompertz model was used to characterize the development of mycelium and spores for each isolate under the same conditions. The lag time, maximal growth rate and total quantity of both extraradical hyphae and spores were determined. Wide variability among isolates was detected, and all growth parameters were significantly altered by fungal isolate. Discriminant analysis differentiated isolates primarily based on the extent of extraradical hyphae produced, yet such differences did not conclusively correspond to phylogenetic relationships among closely related isolates based on partial SSU sequences. Given that the "Rhizophagus" isolates were grown under controlled conditions for many generations, the expression of phenotypic variability could be attributed to genetic differences that are not completely resolved by phylogenetic analysis employing the small ribosomal gene.
在单菌培养条件下，对从阿根廷不同地点分离出的14株“根内根孢囊霉（Rhizophagus）”菌株的根外菌丝生长动态和孢子形成进行了评估。采用改良的Gompertz模型来表征相同条件下各菌株菌丝体和孢子的发育情况。测定了根外菌丝和孢子的延迟期、最大生长速率以及总量。检测到各菌株间存在广泛变异，且所有生长参数均因真菌菌株的不同而显著改变。判别分析主要根据产生的根外菌丝程度区分菌株，但这种差异与基于部分小亚基（SSU）序列的近缘菌株间的系统发育关系并无确凿对应。鉴于“根内根孢囊霉”菌株在受控条件下培养了多代，表型变异的表达可能归因于利用小核糖体基因进行的系统发育分析未完全解析的遗传差异。
