Eldridge Joshua A, Repko Debra, Mumper Russell J
1 Four Tigers LLC , Lexington, Kentucky.
J Med Food. 2014 Oct;17(10):1064-9. doi: 10.1089/jmf.2013.0177. Epub 2014 Jun 3.
The purpose of these studies was to determine if a Büchi Mini Spray Dryer B-290 (Büchi Corporation, New Castle, DE, USA) could be used to prepare blackberry extract powders containing mannitol as a thermoprotectant without extensively degrading anthocyanins and polyphenols in the resulting powders. Three blackberry puree extract samples were each prepared by sonication of puree in 30/70% ethanol/water containing 0.003% HCl. Blackberry puree extract sample 1 (S1) contained no mannitol, while blackberry puree extract sample 2 (S2) contained 3.0:1 (w/w) mannitol:berry extract, and blackberry puree extract sample 3 (S3) contained 6.3:1 (w/w) mannitol:berry extract. The levels of anthocyanins and polyphenols in reconstituted spray-dried powders produced from S1-S3 were compared to solutions of S1-S3 that were held at 4°C as controls. All extract samples could be spray-dried using the Büchi Mini Spray Dryer B-290. S1, with no mannitol, showed a 30.8% decrease in anthocyanins and a 24.1% decrease in polyphenols following spray-drying. However, S2 had a reduction in anthocyanins of only 13.8%, while polyphenols were reduced by only 6.1%. S3, with a ratio of mannitol to berry extract of 6.3:1, exhibited a 12.5% decrease in anthocyanins while the decrease in polyphenols after spray-drying was not statistically significant (P=.16). Collectively, these data indicate that a Büchi Mini Spray Dryer B-290 is a suitable platform for producing stable berry extract powders, and that mannitol is a suitable thermoprotectant that facilitates retention of thermosensitive polyphenolic species in berry extracts during spray-drying.
这些研究的目的是确定Büchi Mini喷雾干燥器B - 290（美国特拉华州纽卡斯尔的Büchi公司）是否可用于制备含有甘露醇作为热保护剂的黑莓提取物粉末，且不会使所得粉末中的花青素和多酚大量降解。通过在含有0.003%盐酸的30/70%乙醇/水体系中对黑莓果泥进行超声处理，分别制备了三个黑莓果泥提取物样品。黑莓果泥提取物样品1（S1）不含甘露醇，而黑莓果泥提取物样品2（S2）含有3.0:1（w/w）的甘露醇:黑莓提取物，黑莓果泥提取物样品3（S3）含有6.3:1（w/w）的甘露醇:黑莓提取物。将由S1 - S3喷雾干燥得到的粉末复溶后，其花青素和多酚水平与作为对照在4°C保存的S1 - S3溶液进行比较。所有提取物样品均可使用Büchi Mini喷雾干燥器B - 290进行喷雾干燥。不含甘露醇的S1在喷雾干燥后花青素含量下降了30.8%，多酚含量下降了24.1%。然而，S2的花青素仅减少了13.8%，多酚仅减少了6.1%。甘露醇与黑莓提取物比例为6.3:1的S3，其花青素含量下降了12.5%，喷雾干燥后多酚含量的下降无统计学意义（P = 0.16）。总体而言，这些数据表明Büchi Mini喷雾干燥器B - 290是生产稳定黑莓提取物粉末的合适平台，且甘露醇是一种合适的热保护剂，有助于在喷雾干燥过程中保留黑莓提取物中热敏性多酚类物质。
