丹麦人群中三个 STR 基因座 D3S1358、D12S391 和 D21S11 的二代测序及测序 STR 等位基因的新命名法
Second generation sequencing of three STRs D3S1358, D12S391 and D21S11 in Danes and a new nomenclature for sequenced STR alleles.
机构信息
Section of Forensic Genetics, Department of Forensic Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Denmark; Faculty of Mathematical, Physical and Natural Sciences, University of Palermo, Palermo, Italy.
Section of Forensic Genetics, Department of Forensic Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Denmark.
出版信息
Forensic Sci Int Genet. 2014 Sep;12:38-41. doi: 10.1016/j.fsigen.2014.04.016. Epub 2014 May 10.
Second generation sequencing (SGS) may revolutionize the field of forensic STR typing. Two of the essential requirements for implementation of an SGS based approach for forensic investigations are (1) establishment of adequate frequency databases and (2) adoption of a new STR nomenclature. We report the STR sequences and allele frequencies of three STR loci: D3S1358, D12S391 and D21S11 in 197 unrelated Danes. We used a new STR nomenclature that depicts the locus name used in forensic genetics, the length of the repeat region divided by the repeat length (typically 4 nucleotides) and detailed sequence information of possible sub-repeats and SNPs within the amplified fragment.
第二代测序（SGS）可能会彻底改变法医 STR 分型领域。在法医调查中实施基于 SGS 的方法有两个基本要求：(1) 建立足够的频率数据库，(2) 采用新的 STR 命名法。我们报告了三个 STR 基因座：D3S1358、D12S391 和 D21S11 在 197 个无关丹麦人个体中的 STR 序列和等位基因频率。我们使用了一种新的 STR 命名法，该命名法描述了法医遗传学中使用的基因座名称、重复区域的长度除以重复长度（通常为 4 个核苷酸）以及扩增片段内可能的亚重复和 SNP 的详细序列信息。
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