Cheng Yanmei, Dang Aimin, Lv Naqiang, Gao Qian, Chen Bingwei, Liu Guozhang
State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100037, People's Republic of China.
Department of Cardiology, Fuwai Hospital and Cardiovascular Institute, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100037, People's Republic of China.
Clin Rheumatol. 2016 Mar;35(3):673-8. doi: 10.1007/s10067-014-2674-3. Epub 2014 Jun 4.
Takayasu arteritis (TA) patients with active disease often have elevated serum C-reactive protein (CRP) levels, which usually decline with the disease remission. The serum CRP concentration has been showed to be related to CRP gene polymorphisms in previous studies. The present study aims to investigate the associations of serum level of CRP and CRP polymorphisms with TA. A total of 178 unrelated Chinese Han TA patients and 229 unrelated Chinese Han individuals without documented disease were enrolled in our studies. After a systemic search in the HapMap database, four single-nucleotide polymorphisms (SNPs) were selected, namely, rs1800947, rs3093077, rs1205, and rs2808630. The ligase detection reaction (LDR) was used in genotyping. CRP concentrations were determined using turbidimetric immunoassay. Genotype frequencies and allele frequencies of CRP variations were similar between TA patients and controls. CRP haplotype frequencies in patients were not significantly different from those of controls. No significant association between serum CRP concentrations and genotypes was found. Moreover, no association was found in CRP concentration between patients with types I, II, and III TA or between patients with or without pulmonary involvement. By contrast, serum CRP concentration was directly correlated with disease severity. In conclusion, CRP polymorphisms were not associated with TA susceptibility or serum CRP levels in the Chinese Han population. However, higher CRP level was correlated with a more serious disease status, which implies that CRP possibly contributes to the progression of TA.
活动性大动脉炎（TA）患者的血清C反应蛋白（CRP）水平通常会升高，而随着疾病缓解，该水平通常会下降。先前的研究表明，血清CRP浓度与CRP基因多态性有关。本研究旨在探讨血清CRP水平和CRP多态性与TA的关联。我们的研究共纳入了178名无亲缘关系的中国汉族TA患者和229名无疾病记录的无亲缘关系的中国汉族个体。在HapMap数据库中进行全面检索后，选择了四个单核苷酸多态性（SNP），即rs1800947、rs3093077、rs1205和rs2808630。采用连接酶检测反应（LDR）进行基因分型。使用比浊免疫分析法测定CRP浓度。TA患者和对照组之间CRP变异的基因型频率和等位基因频率相似。患者的CRP单倍型频率与对照组无显著差异。未发现血清CRP浓度与基因型之间存在显著关联。此外，I型、II型和III型TA患者之间或有或无肺部受累的患者之间，CRP浓度均无关联。相比之下，血清CRP浓度与疾病严重程度直接相关。总之，在中国汉族人群中，CRP多态性与TA易感性或血清CRP水平无关。然而，较高的CRP水平与更严重的疾病状态相关，这意味着CRP可能促进TA的进展。
