肥胖相关妊娠中系统性和胎盘组织瘦素及其受体。
Systemic and placental leptin and its receptors in pregnancies associated with obesity.
机构信息
Department of Obstetrics, Gynecology, and Women's Health, John A. Burns School of Medicine, University of Hawaii, Honolulu, HI, USA
John A. Burns School of Medicine, University of Hawaii, Biostatistic Core, Honolulu, HI, USA.
出版信息
Reprod Sci. 2015 Feb;22(2):189-97. doi: 10.1177/1933719114537718. Epub 2014 Jun 4.
This study aimed to gain new insights into both systemic and placental leptin and its receptors, with reference to the maternal prepregnancy body mass index (BMI). Thus, 84 women (29 lean, 24 overweight, and 31 obese) were recruited and maternal, cord blood, and placental tissues collected prior to term labor. Plasma levels were measured by enzyme-linked immunosorbent assay and for placenta, immunohistochemistry and messenger RNAs (mRNAs) were quantitated. We confirmed that maternal leptin increased linearly as the soluble receptor decreased with BMI (P = .001). Fetal leptin increased with maternal BMI (P = .02) and birth weight (P = .006) and was higher in female infants (P < .001). Placental mRNA levels of leptin and its receptors showed no change in BMI. However, we show a significant (P = .043) linear increase in leptin in the placental vascular endothelial cells with maternal obesity, while leptin in syncytiotrophoblast showed no statistical change. Leptin receptors localized to syncytiotrophoblast and intravillous macrophages and were unchanged with BMI.
本研究旨在深入了解全身和胎盘瘦素及其受体，并参考母体孕前体重指数（BMI）。因此，招募了 84 名妇女（29 名正常体重、24 名超重和 31 名肥胖），在足月分娩前采集了母亲、脐带血和胎盘组织。通过酶联免疫吸附试验测量血浆水平，并对胎盘进行免疫组织化学和信使 RNA（mRNA）定量分析。我们证实，随着可溶性受体的减少，母体瘦素呈线性增加，与 BMI 呈正相关（P =.001）。胎儿瘦素随母体 BMI（P =.02）和出生体重（P =.006）增加而增加，且在女性婴儿中更高（P <.001）。胎盘瘦素及其受体的 mRNA 水平在 BMI 上没有变化。然而，我们发现，随着母体肥胖，胎盘血管内皮细胞中的瘦素呈显著（P =.043）线性增加，而合体滋养层中的瘦素没有统计学变化。瘦素受体定位于合体滋养层和绒毛内巨噬细胞，与 BMI 无关。
Zotero 插件