Department of Civil, Environmental, Aerospace, Materials Engineering, University of Palermo, 90142, Palermo, Italy.
Department of Civil, Environmental, Aerospace, Materials Engineering, University of Palermo, 90142, Palermo, Italy.
Mater Sci Eng C Mater Biol Appl. 2014 Aug 1;41:301-8. doi: 10.1016/j.msec.2014.04.045. Epub 2014 May 2.
In this study a chemical grafting procedure was set up in order to link high molecular weight poly L-lactic acid (PLLA) chains to the hydrophilic α,β-poly(N-2-hydroxyethyl)-DL-aspartamide (PHEA) backbone. A graft copolymer named PHEA-g-PLLA (or simply PHEA-PLLA) was obtained bearing a degree of derivatization of 1.0 mol.% of PLLA as grafted chain. This new hybrid derivative offers both the opportune crystallinity necessary for the production of scaffolds trough a thermally induced phase separation (TIPS) technique and the proper chemical reactivity to perform further functionalizations with bio-effectors and drugs. PHEA-PLLA porous scaffolds for tissue engineering applications were successfully obtained via TIPS and characterized. Structures with an open porosity and a good level of interconnection were detected. As the applicability of the scaffold is mainly dependent on its pore size, preliminary studies about the mechanisms governing scaffold's pore diameter were carried out.
在这项研究中，建立了一种化学接枝工艺，以便将高分子量聚 L-丙交酯（PLLA）链连接到亲水性的α，β-聚（N-2-羟乙基）-DL-天冬酰胺（PHEA）主链上。得到了一种接枝共聚物，命名为 PHEA-g-PLLA（或简称为 PHEA-PLLA），其接枝链的衍生度为 1.0 mol%。这种新型的杂化衍生物具有通过热致相分离（TIPS）技术生产支架所需的适当结晶度，以及与生物效应物和药物进一步进行功能化的适当化学反应性。通过 TIPS 成功获得了用于组织工程应用的 PHEA-PLLA 多孔支架，并对其进行了表征。检测到具有开放孔隙率和良好连接水平的结构。由于支架的适用性主要取决于其孔径，因此进行了关于控制支架孔径的机制的初步研究。
