Croese Elsemiek, Jeremiasse Adriaan W, Marshall Ian P G, Spormann Alfred M, Euverink Gert-Jan W, Geelhoed Jeanine S, Stams Alfons J M, Plugge Caroline M
Laboratory of Microbiology, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, The Netherlands; Wetsus, Centre of Excellence for Sustainable Water Technology, P.O. Box 1113, 8900 CC Leeuwarden, The Netherlands.
Wetsus, Centre of Excellence for Sustainable Water Technology, P.O. Box 1113, 8900 CC Leeuwarden, The Netherlands; Laboratory of Physical Chemistry and Colloid Science, Wageningen University, Dreijenplein 6, P.O. Box 8038, 6700 EK Wageningen, The Netherlands; Sub-Department of Environmental Technology, Wageningen University, Bomenweg 2, P.O. Box 8129, 6700 EV Wageningen, The Netherlands.
Enzyme Microb Technol. 2014 Jul-Aug;61-62:67-75. doi: 10.1016/j.enzmictec.2014.04.019. Epub 2014 May 9.
The microbial electrolysis cell (MEC) biocathode has shown great potential as alternative for expensive metals as catalyst for H2 synthesis. Here, the bacterial communities at the biocathode of five hydrogen producing MECs using molecular techniques were characterized. The setups differed in design (large versus small) including electrode material and flow path and in carbon source provided at the cathode (bicarbonate or acetate). A hydrogenase gene-based DNA microarray (Hydrogenase Chip) was used to analyze hydrogenase genes present in the three large setups. The small setups showed dominant groups of Firmicutes and two of the large setups showed dominant groups of Proteobacteria and Bacteroidetes. The third large setup received acetate but no sulfate (no sulfur source). In this setup an almost pure culture of a Promicromonospora sp. developed. Most of the hydrogenase genes detected were coding for bidirectional Hox-type hydrogenases, which have shown to be involved in cytoplasmatic H2 production.
微生物电解池（MEC）生物阴极作为用于H2合成的催化剂，已显示出作为昂贵金属替代品的巨大潜力。在此，使用分子技术对五个产氢MEC生物阴极处的细菌群落进行了表征。这些装置在设计（大型与小型）上存在差异，包括电极材料和流动路径，并且在阴极提供的碳源（碳酸氢盐或乙酸盐）方面也有所不同。基于氢化酶基因的DNA微阵列（氢化酶芯片）用于分析三个大型装置中存在的氢化酶基因。小型装置显示厚壁菌门占主导地位，两个大型装置显示变形菌门和拟杆菌门占主导地位。第三个大型装置接受乙酸盐但不接受硫酸盐（无硫源）。在这个装置中，一种原小单孢菌属的几乎纯培养物得以生长。检测到的大多数氢化酶基因编码双向Hox型氢化酶，这些氢化酶已被证明参与细胞质H2的产生。
