Imam Hasan, Chowdhury Arfia, Mahbub Nasir Uddin, Hossain Amir, Karim Mohammed Faisal Bin, Uddin Mohammad Burhan, Sarker Md Moklesur Rahman
Department of Pharmacy, Primeasia University, 9 Banani C/A, Dhaka 1213, Bangladesh.
Exim Bank Hospital, 840 Rokeya Soroni, Mirpur, Dhaka 1216, Bangladesh.
J Diabetes Metab Disord. 2014 May 1;13:54. doi: 10.1186/2251-6581-13-54. eCollection 2014.
Present study describe the in vitro model of plasma oxidation of patients with different lipid profile, that can be correlated to their invivo plasma oxidizability in order to find the arterial diseases prone patient groups.
The method applied here to measure the invitro plasma oxidizability, accounts a convenient way that can be well suited in any clinical laboratory settings. Un-fractionated plasma was exposed to CuSO4 (5.0 mmol/L), a pro-oxidant, and low frequency ultrasonic wave to induce oxidation, and finally oxidizability was calculated by TBARS and Conjugated Diene methods.
In our study, plasma LDL greater than 150 mg/dL possess 1.75 times more risk to undergo oxidation (CI, 0.7774 to 3.94; p = 0.071) than the low LDL plasma, percent of oxidation increased from 38.3% to 67.1% for the LDL level upto 150 mg/dL and high. Lag phase, which is considered as the plasma antioxidative protection, was also influenced by the higher LDL concentration. The mean lag time was 65.27 ± 20.02 (p = 0.02 compared to healthy), where as for 94.71 ± 35.11 min for the normolipidemic subject. The plasma oxidizability was also changed drastically for total cholesterol level, oxidative susceptibility shown 35% and 55.02% for 200 mg/dL and high respectively, however it didn't appear as risk factor. Patient samples were also stratified according to their age, gender, and blood glucose level. Older persons (≥40 years) were 1.096 times (95% CL, 0.5607 to 2.141, p = 0.396) than younger (≤39 years age), males are 1.071 (95% CI, 0.5072- 2.264) times than the females, and diabetic patients are 1.091 (CI, 0.6153 to 1.934, p = 0.391) times in more risk than the non-diabetic counterpart.
This method addressing its easy applicability in biomedical research. And by this we were able to show that patients with high LDL (≥150 mg/dL) are in alarming condition besides diabetic and elderly (≥40 years age) males are considered to be susceptible and more prone to develop vascular diseases.
本研究描述了具有不同血脂水平患者的血浆氧化体外模型，该模型可与其体内血浆氧化能力相关联，以便找出易患动脉疾病的患者群体。
此处应用的测量体外血浆氧化能力的方法，是一种适用于任何临床实验室环境的便捷方法。将未分级的血浆暴露于促氧化剂硫酸铜（5.0 mmol/L）和低频超声波以诱导氧化，最后通过硫代巴比妥酸反应物（TBARS）和共轭二烯方法计算氧化能力。
在我们的研究中，血浆低密度脂蛋白（LDL）大于150 mg/dL的患者发生氧化的风险比血浆LDL水平低的患者高1.75倍（置信区间，0.7774至3.94；p = 0.071），LDL水平高达150 mg/dL及以上时，氧化百分比从38.3%增加到67.1%。被视为血浆抗氧化保护的滞后期也受到较高LDL浓度的影响。平均滞后期为65.27±20.02（与健康人相比，p = 0.02），而血脂正常者为94.71±35.11分钟。血浆氧化能力也因总胆固醇水平而发生显著变化，氧化敏感性在总胆固醇水平为200 mg/dL及以上时分别为35%和55.02%，然而它并未表现为风险因素。患者样本还根据年龄、性别和血糖水平进行了分层。老年人（≥40岁）比年轻人（≤39岁）发生氧化的风险高1.096倍（95%置信区间，0.5607至2.141，p = 0.396），男性比女性高1.071倍（95%置信区间，0.5072至2.264），糖尿病患者比非糖尿病患者发生氧化的风险高1.091倍（置信区间，0.6153至1.934，p = 0.391）。
该方法在生物医学研究中易于应用。通过此方法我们能够表明，除糖尿病患者外，LDL水平高（≥150 mg/dL）的患者处于令人担忧的状态，并且老年男性（≥40岁）被认为易患且更倾向于患血管疾病。
