Oral Biotechnology and Bioengineering, Department of Cranio-maxillofacial and Oral Surgery, University Hospital Zurich, and Center for Dental Medicine, University of Zurich, Switzerland.
Zurich Centre for Integrative Human Physiology, University of Zurich, Switzerland.
J Tissue Eng Regen Med. 2017 Feb;11(2):425-433. doi: 10.1002/term.1926. Epub 2014 Jun 12.
Guided bone regeneration (GBR) has been utilized for several decades for the healing of cranio-maxillofacial bone defects and, particularly in the dental field, by creating space with a barrier membrane to exclude soft tissue and encourage bone growth in the membrane-protected volume. Although the first membranes were non-resorbable, a new generation of GBR membranes aims to biodegrade and provide bioactivity for better overall results. The Inion GTR™ poly(lactide-co-glycolide) (PLGA) membrane is not only resorbable but also bioactive, since it includes N-methylpyrrolidone (NMP), which has been shown to promote bone regeneration. In this study, the effects of loading different amounts of NMP onto the membrane through chemical vapour deposition or dipping have been explored. In vitro release demonstrated that lower levels of NMP led to lower NMP concentrations and slower release, based on total NMP loaded in the membrane. The dipped membrane released almost all of the NMP within 15 min, leading to a high NMP concentration. For the in vivo studies in rabbits, 6 mm calvarial defects were created and left untreated or covered with an ePTFE membrane or PLGA membranes dipped in, or preloaded with, NMP. Evaluation of the bony regeneration revealed that the barrier membranes improved bony healing and that a decrease in NMP content improved the performance. Overall, we have demonstrated the potential of these PLGA membranes with a more favourable NMP release profile and the significance of exploring the effect of NMP on these PLGA membranes with regard to bone ingrowth. Copyright © 2014 John Wiley & Sons, Ltd.
引导骨再生（GBR）已被用于治疗颅颌面骨缺损数十年，特别是在牙科领域，通过使用屏障膜创建空间，以排除软组织并促进膜保护体积中的骨生长。尽管最初的膜是不可吸收的，但新一代 GBR 膜旨在生物降解并提供生物活性，以获得更好的整体效果。Inion GTR™聚（乳酸-共-乙醇酸）（PLGA）膜不仅可吸收，而且具有生物活性，因为它包含 N-甲基吡咯烷酮（NMP），已证明 NMP 可促进骨再生。在这项研究中，通过化学气相沉积或浸泡探索了在膜上加载不同量 NMP 的效果。体外释放表明，基于膜中总 NMP 负载量，较低水平的 NMP 导致较低的 NMP 浓度和较慢的释放。浸泡膜在 15 分钟内几乎释放了所有的 NMP，导致 NMP 浓度很高。对于兔的体内研究，创建了 6mm 颅骨缺损，未处理或用 ePTFE 膜或浸泡在 NMP 中的 PLGA 膜或预先加载 NMP 的 PLGA 膜覆盖。对骨再生的评估表明，屏障膜改善了骨愈合，而 NMP 含量的降低改善了性能。总体而言，我们展示了这些具有更有利的 NMP 释放曲线的 PLGA 膜的潜力，并且探索 NMP 对这些 PLGA 膜在骨向内生长方面的影响具有重要意义。版权所有©2014 年 John Wiley & Sons, Ltd.
