Deffains Marc, Holland Peter, Moshel Shay, Ramirez de Noriega Fernando, Bergman Hagai, Israel Zvi
Department of Medical Neurobiology, The Hebrew University-Hadassah Medical School, Jerusalem, Israel; The Edmond and Lily Safra Center for Brain Sciences, The Hebrew University, Jerusalem, Israel;
Alpha Omega Engineering Ltd., Nazareth, Israel;
J Neurophysiol. 2014 Sep 15;112(6):1409-20. doi: 10.1152/jn.00170.2014. Epub 2014 Jun 11.
In Parkinson's disease, pathological synchronous oscillations divide the subthalamic nucleus (STN) of patients into a dorsolateral oscillatory region and ventromedial nonoscillatory region. This bipartite division reflects the motor vs. the nonmotor (associative/limbic) subthalamic areas, respectively. However, significant topographic differences in the neuronal discharge rate between these two STN subregions in Parkinsonian patients is still controversial. In this study, 119 STN microelectrode trajectories (STN length > 2 mm, mean = 5.32 mm) with discernible oscillatory and nonoscillatory regions were carried on 60 patients undergoing deep brain stimulation surgery for Parkinson's disease. 2,137 and 2,152 multiunit stable signals were recorded (recording duration > 10 s, mean = 21.25 s) within the oscillatory and nonoscillatory STN regions, respectively. Spike detection and sorting were applied offline on every multiunit stable signal using an automatic method with systematic quantification of the isolation quality (range = 0-1) of the identified units. In all, 3,094 and 3,130 units were identified in the oscillatory and nonoscillatory regions, respectively. On average, the discharge rate of better-isolated neurons (isolation score > 0.70) was higher in the oscillatory region than the nonoscillatory region (44.55 ± 0.87 vs. 39.97 ± 0.77 spikes/s, N = 665 and 761, respectively). The discharge rate of the STN neurons was positively correlated to the strength of their own and their surrounding 13- to 30-Hz beta oscillatory activity. Therefore, in the Parkinsonian STN, beta oscillations and higher neuronal discharge rate are correlated and coexist in the motor area of the STN compared with its associative/limbic area.
在帕金森病中，病理性同步振荡将患者的丘脑底核（STN）分为背外侧振荡区域和腹内侧非振荡区域。这种二分法分别反映了丘脑底核的运动区域与非运动（联合/边缘）区域。然而，帕金森病患者这两个STN子区域之间神经元放电率的显著地形差异仍存在争议。在本研究中，对60例接受帕金森病深部脑刺激手术的患者进行了119条STN微电极轨迹（STN长度>2 mm，平均=5.32 mm），这些轨迹具有可辨别的振荡和非振荡区域。分别在振荡和非振荡的STN区域记录了2137个和2152个多单元稳定信号（记录持续时间>10 s，平均=21.25 s）。使用自动方法对每个多单元稳定信号进行离线尖峰检测和分类，并系统量化识别单元的分离质量（范围=0-1）。总共在振荡区域和非振荡区域分别识别出3094个和3130个单元。平均而言，振荡区域中分离较好的神经元（分离分数>0.70）的放电率高于非振荡区域（分别为44.55±0.87和39.97±0.77个尖峰/秒，N分别为665和761）。STN神经元的放电率与其自身以及周围13至30 Hz的β振荡活动强度呈正相关。因此，在帕金森病的STN中，与联合/边缘区域相比，β振荡和较高的神经元放电率在STN的运动区域相关联并共存。
