Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, 836 Ohya, Shizuoka 422-8529, Japan.
Department of Animal Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan.
Biosci Microbiota Food Health. 2013;32(4):149-56. doi: 10.12938/bmfh.32.149. Epub 2013 Oct 30.
This study aimed to examine the mechanism for differential effects of low- (LPPS) and high-phosphorus (HPPS) potato starches and high-amylose cornstarch (HACS) on rat cecal fermentation, the n-butyrate proportion in particular. In ileorectostomized rats, the in vivo resistant starch (RS) contents were determined to be 66% (LPPS), 66% (HPPS) and 36% (HACS), but the carbohydrate/nitrogen (C/N) ratios of the ileal digesta were comparable among the respective starch diets. In intact rats fed diets including similar amounts of RS, the cecal n-butyrate proportions in the LPPS- and HPPS-fed rats were equally higher than in the HACS-fed rats. The cecal starch contents were fivefold greater in the LPPS- and HPPS-fed rats than in the HACS-fed rats. The results suggest that potato starches and HACS are not equivalent n-butyrate producers in the rat cecum and that the slower fermentation rate of potato starches relative to HACS might be responsible for the higher n-butyrate proportion.
本研究旨在探讨低磷（LPPS）和高磷（HPPS）马铃薯淀粉和高直链玉米淀粉（HACS）对大鼠盲肠发酵的差异作用机制，特别是丁酸比例。在回肠造口大鼠中，体内抗性淀粉（RS）含量分别为 66%（LPPS）、66%（HPPS）和 36%（HACS），但各淀粉饮食的回肠消化物的碳水化合物/氮（C/N）比值相当。在给予含有相似 RS 量饮食的完整大鼠中，LPPS 和 HPPS 喂养大鼠的盲肠丁酸比例均明显高于 HACS 喂养大鼠。LPPS 和 HPPS 喂养大鼠的盲肠淀粉含量是 HACS 喂养大鼠的五倍。结果表明，在大鼠盲肠中，马铃薯淀粉和 HACS 并不是等效的丁酸产生者，相对于 HACS，马铃薯淀粉的发酵速度较慢，可能是丁酸比例较高的原因。
