重组亲和素2融合蛋白的表达、纯化及固定化
Expression, purification, and immobilization of recombinant tamavidin 2 fusion proteins.
作者信息
Takakura Yoshimitsu, Oka Naomi, Tsunashima Masako
机构信息
Plant Innovation Center, Japan Tobacco, Inc., 700 Higashibara, Iwata, Shizuoka, 438-0802, Japan,
出版信息
Methods Mol Biol. 2014;1177:95-106. doi: 10.1007/978-1-4939-1034-2_8.
Tamavidin 2 is a fungal avidin-like protein that binds biotin with high affinity. Unlike avidin or streptavidin, tamavidin 2 in soluble form is produced at high levels in Escherichia coli. In this chapter, we describe a method for immobilization and purification of recombinant proteins with the use of tamavidin 2 as an affinity tag. The protein fused to tamavidin 2 is tightly immobilized and simultaneously purified on biotinylated magnetic microbeads without loss of activity.
亲和素样蛋白2是一种能与生物素高亲和力结合的真菌抗生物素蛋白。与抗生物素蛋白或链霉抗生物素蛋白不同，可溶性的亲和素样蛋白2能在大肠杆菌中高水平表达。在本章中，我们描述了一种利用亲和素样蛋白2作为亲和标签来固定化和纯化重组蛋白的方法。与亲和素样蛋白2融合的蛋白会紧密固定在生物素化的磁性微珠上，同时被纯化，且活性不会丧失。
Zotero 插件