两种二硫键毒素的高通量生产。

High-throughput production of two disulphide-bridge toxins.

作者信息

Upert Grégory, Mourier Gilles, Pastor Alexandra, Verdenaud Marion, Alili Doria, Servent Denis, Gilles Nicolas

机构信息

CEA, DSV, iBiTec-S, Service d'Ingénierie Moléculaire des Protéines (SIMOPRO), CEA Saclay, Gif sur Yvette F-91191, France.

出版信息

Chem Commun (Camb). 2014 Aug 7;50(61):8408-11. doi: 10.1039/c4cc02679a.

DOI:10.1039/c4cc02679a

PMID:24947561

Abstract

A quick and efficient production method compatible with high-throughput screening was developed using 36 toxins belonging to four different families of two disulphide-bridge toxins. Final toxins were characterized using HPLC co-elution, CD and pharmacological studies.

摘要

利用属于两个二硫键毒素四个不同家族的36种毒素，开发了一种与高通量筛选兼容的快速高效生产方法。最终毒素通过HPLC共洗脱、圆二色光谱和药理学研究进行表征。