Santhamani Ramasamy, Yogisharadhya Revanaiah, Venkatesan Gnanavel, Shivachandra Sathish Bhadravati, Pandey Awadh Bihari, Ramakrishnan Muthannan Andavar
Division of Virology, Indian Veterinary Research Institute, Mukteswar, 263 138, Uttarakhand, India.
Virus Genes. 2014 Oct;49(2):286-91. doi: 10.1007/s11262-014-1095-3. Epub 2014 Jun 22.
Sheeppox and goatpox are economically important diseases of small ruminants caused by sheeppox virus (SPPV) and goatpox virus (GTPV), respectively. Although SPPV and GTPV have host preference, some strains may infect both sheep and goats. As capripox viruses (SPPV, GTPV and LSDV) are antigenically related but genetically distinct, their differentiation requires analysis at molecular level. In the present study, RPO30 and GPCR genes of eight Indian SPPV and GTPV isolates were PCR amplified, cloned and sequences are genetically and phylogenetically analyzed. The RPO30 gene of SPPV and GTPV had lineage-specific signatures, and deletion of 21-nucleotide exclusively present in SPPV. Similarly, GPCR gene also had lineage-specific signatures for SPPV and GTPV. Phylogenetic analysis of capripox viruses based on RPO30 and GPCR genes revealed three distinct lineage-specific clusters as per their host origin. Our study supports that both RPO30 and GPCR genes could be used for differentiation of SPPV and GTPV as well as for molecular epidemiological studies. The study also highlights the distinct lineage specificities of the Indian SPPV and GTPV isolates including vaccine strains.
绵羊痘和山羊痘分别是由绵羊痘病毒（SPPV）和山羊痘病毒（GTPV）引起的对小型反刍动物具有重要经济意义的疾病。虽然SPPV和GTPV具有宿主偏好性，但一些毒株可能同时感染绵羊和山羊。由于山羊痘病毒（SPPV、GTPV和LSDV）在抗原上相关但基因上不同，它们的区分需要在分子水平上进行分析。在本研究中，对8株印度SPPV和GTPV分离株的RPO30和GPCR基因进行了PCR扩增、克隆，并对序列进行了遗传和系统发育分析。SPPV和GTPV的RPO30基因具有谱系特异性特征，且存在仅在SPPV中出现的21个核苷酸的缺失。同样，GPCR基因对SPPV和GTPV也具有谱系特异性特征。基于RPO30和GPCR基因对山羊痘病毒进行的系统发育分析显示，根据其宿主来源可分为三个不同的谱系特异性簇。我们的研究支持RPO30和GPCR基因均可用于区分SPPV和GTPV以及进行分子流行病学研究。该研究还突出了包括疫苗株在内的印度SPPV和GTPV分离株独特的谱系特异性。
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