Zhang Liang, Lei Yang, Liu Xia, Wang Xiao, Liu Zhao, Li Dan, Zheng Peng, Zhang Lujun, Chen Shigang, Xie Peng
Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China; Institute of Neuroscience, Chongqing Medical University, Chongqing, China; Chongqing Key Laboratory of Neurobiology, Chongqing Medical University, Chongqing, China.
Institute of Neuroscience, Chongqing Medical University, Chongqing, China; Chongqing Key Laboratory of Neurobiology, Chongqing Medical University, Chongqing, China; Department of Neurology, University-Town Hospital of Chongqing Medical University, Chongqing, China.
PLoS One. 2014 Jun 23;9(6):e99752. doi: 10.1371/journal.pone.0099752. eCollection 2014.
Borna disease virus (BDV) is a neurotropic, enveloped, non-segmented, negative-stranded RNA virus that infects a wide variety of vertebrate species from birds to humans across a broad global geographic distribution. Animal symptomatology range from asymptomatic infection to behavioral abnormalities to acute meningoencephalitis. Asymptomatic BDV infection has been shown to be more frequent than conventionally estimated. However, the molecular mechanism(s) underyling asymptomatic BDV infection remain largely unknown. Here, based on real-time quantitative PCR and Western blotting, a total of 18 horse hippocampi were divided into BDV-infected (n = 8) and non-infected control (n = 10) groups. A gas chromatography coupled with mass spectrometry (GC-MS) metabolomic approach, in conjunction with multivariate statistical analysis, was used to characterize the hippocampal metabolic changes associated with asymptomatic BDV infection. Multivariate statistical analysis showed a significant discrimination between the BDV-infected and control groups. BDV-infected hippocampi were characterized by lower levels of D-myo-inositol-1-phosphate, glutamate, phosphoethanolamine, heptadecanoic acid, and linoleic acid in combination with a higher level of ammonia. These differential metabolites are primarily involved in glutamate and lipid metabolism. These finding provide an improved understanding of hippocampal changes associated with asymptomatic BDV infection.
博尔纳病病毒（BDV）是一种嗜神经性、有包膜、不分节段的负链RNA病毒，在全球广泛的地理分布范围内感染从鸟类到人类的多种脊椎动物。动物的症状表现从无症状感染到行为异常，再到急性脑膜脑炎。已证明无症状BDV感染比传统估计更为常见。然而，无症状BDV感染背后的分子机制在很大程度上仍然未知。在此，基于实时定量PCR和蛋白质印迹法，将总共18个马海马体分为BDV感染组（n = 8）和未感染对照组（n = 10）。采用气相色谱-质谱联用（GC-MS）代谢组学方法，并结合多变量统计分析，来表征与无症状BDV感染相关的海马体代谢变化。多变量统计分析显示BDV感染组和对照组之间存在显著差异。BDV感染的海马体特征在于D-肌醇-1-磷酸、谷氨酸、磷酸乙醇胺、十七烷酸和亚油酸水平较低，同时氨水平较高。这些差异代谢物主要参与谷氨酸和脂质代谢。这些发现有助于更好地理解与无症状BDV感染相关的海马体变化。
