Xiong Yong-xing, Wu Lan, Liu Yi-mei, Chen Ke-li, Xiong Yong-xing, Wu Lan, Liu Yi-mei, Chen Ke-li
Zhong Yao Cai. 2013 Nov;36(11):1762-5.
To identify Peucedani Radix and its adulterants using DNA barcoding technique.
Total genomic DNA was isolated from Peucedani Radix and its adulterants. Nuclear DNA ITS2 sequences were amplified and purified PCR products were sequenced. Sequence assembly and consensus sequence generation were performed using the CodonCode Aligner V3.0. The Kimura 2-Parameter(K2P) distances were calculated using software MEGA 4. 0. Identification analyses were performed using BLAST1, Nearest Distance and Neighbor-Joining (NJ) methods, and the secondary structure of the ITS2 sequence differences between species were analyzed.
Different samples of Peucedani Radix were gathered together and distinguished from its adulterants by NJ tree. The ITS2 secondary structure showed that Peucedani Radix could be differentiated obviously from its adulterants.
ITS2 sequence is able to identify Peucedani Radix and its adulterants correctly, which provides a scientific basis for fast and accurate identification of the herb.
采用DNA条形码技术鉴别前胡及其伪品。
从前胡及其伪品中提取总基因组DNA。扩增核DNA ITS2序列，对纯化后的PCR产物进行测序。使用CodonCode Aligner V3.0进行序列组装和一致性序列生成。使用MEGA 4.0软件计算Kimura双参数（K2P）距离。采用BLAST1、最近距离和邻接法（NJ）进行鉴定分析，并分析物种间ITS2序列差异的二级结构。
通过NJ树将不同的前胡样品聚集在一起，并与伪品区分开来。ITS2二级结构表明，前胡与其伪品能够明显区分。
ITS2序列能够正确鉴别前胡及其伪品，为该药材的快速准确鉴别提供了科学依据。
Zotero 插件