Lin Han-Lin, Tsao Hsiang-Wei, Huang Yu-Wen, Wang Yi-Chuan, Yang Keng-Hao, Yang Ya-Fei, Wang Wei-Chiang, Wen Chun-Kuei, Chen Sheng-Kun, Cheng Sheng-Shung
Department of Environmental Engineering, National Cheng Kung University, No. 1, University Rd, Tainan 70101, Taiwan E-mail:
Department of Environmental Engineering, Kun Shan University, No. 949, Dawan Rd, Tainan 71003, Taiwan.
Water Sci Technol. 2014;69(12):2526-32. doi: 10.2166/wst.2014.170.
A laboratory study was undertaken to explore the capability of one-stage ANAMMOX in a hybrid biofilm-carrier reactor (HBCR) fed with petrochemical wastewater. Under favorable operating conditions in continuous-flow operations (at the dissolved oxygen level of 0.5-1.0 mg L(-1)), the average total nitrogen (TN) removal efficiency reached 62-67% and approximately 90% of TN can be removed by ANAMMOX. In batch operations of the hybrid biofilm-carrier reactor (without adding carbon substrate), the specific TN removal rate of the reactor in which both Kaldnes and nonwoven carriers were kept was two-fold higher than that of the reactor in which only nonwoven carriers were kept. This indicated that the microbial activity of thinner biofilms (Kaldnes carriers) was remarkably higher than that of thicker biofilms (nonwoven carriers). Finally, based on the 16S rRNA clone library, a cluster of ANAMMOX Candidatus Kuenenia stuttgartiensis was identified.
开展了一项实验室研究,以探究在以石化废水为进水的混合生物膜载体反应器(HBCR)中进行单级厌氧氨氧化的能力。在连续流操作的有利运行条件下(溶解氧水平为0.5 - 1.0 mg L(-1)),平均总氮(TN)去除效率达到62 - 67%,且约90%的TN可通过厌氧氨氧化去除。在混合生物膜载体反应器的批次操作中(不添加碳底物),同时保留了Kaldnes载体和非织造载体的反应器的比TN去除率比仅保留非织造载体的反应器高出两倍。这表明较薄生物膜(Kaldnes载体)的微生物活性明显高于较厚生物膜(非织造载体)。最后,基于16S rRNA克隆文库,鉴定出了厌氧氨氧化候选库氏斯图加特菌簇。