Mihalache Daniela, Giuşcă Simona Eliza, Balan Raluca, Amălinei Cornelia, Grigoraş Adriana, Căruntu Irina Draga
Department of Morphofunctional Sciences, "Grigore T. Popa" University of Medicine and Pharmacy, Iassy, Romania;
Rom J Morphol Embryol. 2014;55(2):273-7.
This study focuses on the analysis of geometric descriptors that can be applied in breast cytology, and their correlation with the qualitative features, with the aim to underline the differences between the benign and malignant cell profile. The morphometric investigation was performed on smears obtained by fine needle aspiration, 10 cases (group 1) diagnosed as benign and 10 cases (group 2) as malignant. For group 2, the malignancy was histopathologically confirmed on the surgical resection specimen. The sequence of automated operation, previously reported by us, permitted the extraction of the following geometrical descriptors: cytoplasmic area, nuclear area, nucleo-cytoplasmic ratio, equivalent diameter and form factor. We analyzed the differences between the benign and malignant morphometric features, and the correlation between the malignant morphometric features and cytological, respectively histological grading. Statistically significant difference in cytoplasmic areas, nuclear areas, value of nucleo-cytoplasmic ratio and equivalent diameter was noted between group I and II. For the form factor, we did not register statistically significant differences. For group 2, the correlation between the morphometric features and cytological grading revealed that the nuclear area is the most valuable descriptor, due to the significant differences between the three successive grades of cytological severity, followed by the cytoplasmic area and equivalent diameter, their numerical values presenting significant differences between cytological grade 1 and 3, and 2 and 3, respectively. The statistical analysis between the morphometric features and histological grading showed that nuclear area and equivalent diameter are the most viable indicators, due to the significant differences present between the three successive grades of pathologic severity, followed by cytoplasmic area (significant differences only for grade 2 versus 3) and for nucleo-cytoplasmic ratio (significant differences only for grade 1 versus 2). The form factor does not provide information that could be correlated with the cytological or histological grading. The defined morphometric features enable the characterization of benign and malignant cells and provide objective criteria that could support a differentiation of benign from the malignant pathology in the cytological diagnosis.
本研究聚焦于可应用于乳腺细胞学的几何描述符分析，以及它们与定性特征的相关性，旨在强调良性和恶性细胞形态之间的差异。对通过细针穿刺获得的涂片进行形态计量学研究，其中10例（第1组）诊断为良性，10例（第2组）诊断为恶性。对于第2组，手术切除标本经组织病理学证实为恶性。我们之前报道的自动操作序列允许提取以下几何描述符：细胞质面积、细胞核面积、核质比、等效直径和形状因子。我们分析了良性和恶性形态计量学特征之间的差异，以及恶性形态计量学特征与细胞学分级和组织学分级之间的相关性。第I组和第II组在细胞质面积、细胞核面积、核质比值和等效直径方面存在统计学显著差异。对于形状因子，我们未记录到统计学显著差异。对于第2组，形态计量学特征与细胞学分级之间的相关性表明，细胞核面积是最有价值的描述符，因为在细胞学严重程度的三个连续等级之间存在显著差异，其次是细胞质面积和等效直径，它们的数值在细胞学1级和3级之间以及2级和3级之间分别存在显著差异。形态计量学特征与组织学分级之间的统计分析表明，细胞核面积和等效直径是最可行的指标。因为在病理严重程度的三个连续等级之间存在显著差异，其次是细胞质面积（仅在2级与3级之间存在显著差异）和核质比（仅在1级与2级之间存在显著差异）。形状因子未提供可与细胞学或组织学分级相关的信息。所定义的形态计量学特征能够对良性和恶性细胞进行表征，并提供客观标准，可支持在细胞学诊断中区分良性与恶性病变。
