基于RNA测序的熊果酸和白藜芦醇对耐甲氧西林金黄色葡萄球菌生物膜抑制作用的转录组分析
RNA-Seq-based transcriptome analysis of methicillin-resistant Staphylococcus aureus biofilm inhibition by ursolic acid and resveratrol.
作者信息
Qin Nan, Tan Xiaojuan, Jiao Yinming, Liu Lin, Zhao Wangsheng, Yang Shuang, Jia Aiqun
机构信息
1] State Key Laboratory for Diagnosis and Treatment of Infectious Disease, the First Affiliated Hospital, Zhejiang University, Hangzhou 310003, China [2].
1] School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China [2].
出版信息
Sci Rep. 2014 Jun 27;4:5467. doi: 10.1038/srep05467.
Bacterial biofilms are particularly problematic since they become resistant to most available antibiotics. Hence, novel potential antagonists to inhibit biofilm formation are urgent. Here the influences of two natural products, ursolic acid and resveratrol, on biofilm of the clinical methicillin-resistant Staphylococcus aureus (MRSA) isolate were investigated using RNA-seq, and the differentially expressed genes were analyzed using Cuffdiff. The results showed that ursolic acid inhibition of biofilm formation may reduce amino acids metabolism and adhesins expression and resveratrol may disturb quorum sensing (QS) and the synthesis of surface proteins and capsular polysaccharides. In addition, the transcriptome analysis of resveratrol and the combination of resveratrol with vancomycin inhibition of established biofilm revealed that resveratrol would disturb the expression of genes related to QS, surface and secreted proteins, and capsular polysaccharides. These findings suggest that ursolic acid and resveratrol could be useful to be adjunct therapies for the treatment of MRSA biofilm-involved infections.
细菌生物膜尤其成问题，因为它们对大多数现有抗生素产生耐药性。因此，迫切需要新型的潜在拮抗剂来抑制生物膜形成。在此，使用RNA测序研究了两种天然产物熊果酸和白藜芦醇对临床耐甲氧西林金黄色葡萄球菌（MRSA）分离株生物膜的影响，并使用Cuffdiff分析差异表达基因。结果表明，熊果酸对生物膜形成的抑制作用可能会降低氨基酸代谢和黏附素表达，而白藜芦醇可能会干扰群体感应（QS）以及表面蛋白和荚膜多糖的合成。此外，白藜芦醇以及白藜芦醇与万古霉素联合对已形成生物膜的抑制作用的转录组分析表明，白藜芦醇会干扰与QS、表面和分泌蛋白以及荚膜多糖相关的基因表达。这些发现表明，熊果酸和白藜芦醇可能作为辅助疗法用于治疗涉及MRSA生物膜的感染。
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