通过聚合酶链反应-变性梯度凝胶电泳和荧光原位杂交跟踪液态剩余污泥稳定化过程中的微生物群落组成和优势成分。
Tracking the composition and dominant components of the microbial community via polymerase chain reaction-denaturing gradient gel electrophoresis and fluorescence in situ hybridization during vermiconversion for liquid-state excess sludge stabilization.
机构信息
State Key Laboratory of Pollution Control and Resources Reuse, 1239 Siping Road, Shanghai, China; The Institute of Biofilm Technology, Key Laboratory of Yangtze River Water Environment, Ministry of Education, 1239 Siping Road, Shanghai,China; College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China.
State Key Laboratory of Pollution Control and Resources Reuse, 1239 Siping Road, Shanghai, China; The Institute of Biofilm Technology, Key Laboratory of Yangtze River Water Environment, Ministry of Education, 1239 Siping Road, Shanghai,China; College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China.
出版信息
Bioresour Technol. 2014 Sep;167:100-7. doi: 10.1016/j.biortech.2014.05.109. Epub 2014 Jun 5.
To quantitatively explore the microbial community modified by earthworms, a vermifilter (VF, with earthworms) and a conventional biofilter (BF, without earthworms) were continuously operated to stabilize excess sludge. The results demonstrated a positive role imposed by earthworms on compositions and dominant components of microbial community in the VF. For one thing, the phyla Actinobacteria and Acidobacteria were only detected in the VF, which might explain for the higher Shannon index of bacteria in the VF (H = 2.58) than that in the BF (H = 1.99). For another, the total proportion of dominant bacteria in the VF increased by 23% compared to the BF. Moreover, quantification analysis explicitly noted that the dominant bacteria in VF were β-proteobacteria (27 ± 2%) and γ-proteobacteria (24 ± 1%) while that in BF was Bacteroidetes (21 ± 1%). In conclusion, stimulated by earthworms, a unique microbial community developed in the VF, thus improving the stabilization of excess sludge.
为定量探索蚯蚓对微生物群落的改造作用，采用连续运行的蚯蚓滤池（VF，含蚯蚓）和传统生物滤池（BF，不含蚯蚓）稳定剩余污泥。结果表明，蚯蚓对 VF 中微生物群落的组成和优势组分有积极作用。一方面，放线菌门和酸杆菌门仅在 VF 中检测到，这可能解释了 VF 中细菌的 Shannon 指数（H = 2.58）高于 BF（H = 1.99）的原因。另一方面，VF 中优势细菌的总比例比 BF 增加了 23%。此外，定量分析明确指出，VF 中的优势细菌为β-变形菌（27 ± 2%）和γ-变形菌（24 ± 1%），而 BF 中的优势细菌为拟杆菌门（21 ± 1%）。总之，蚯蚓的刺激作用使 VF 中形成了独特的微生物群落，从而提高了剩余污泥的稳定性。
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