Basal Medical College of Nanyang Medical University, Nanyang, 473041 Henan Province, China.
Basal Medical College of Nanyang Medical University, Nanyang, 473041 Henan Province, China.
Gene. 2014 Sep 1;547(2):338-45. doi: 10.1016/j.gene.2014.06.045. Epub 2014 Jun 25.
Hyaluronidase is a common component of scorpion venom and has been considered as "spreading factor" that promotes a fast penetration of the venom in the anaphylactic reaction. In the current study, a novel full-length of hyaluronidase BmHYI and three noncoding isoforms of BmHYII, BmHYIII and BmHYIV were cloned by using a combined strategy based on peptide sequencing and Rapid Amplification of cDNA Ends (RACE). BmHYI has 410 amino acid residues containing the catalytic, positional and five potential N-glycosylation sites. The deduced protein sequence of BmHYI shares significant identity with venom hyaluronidases from bees and snakes. The phylogenetic analysis showed early divergence and independent evolution of BmHYI from other hyaluronidases. An extraordinarily high level of sequence similarity was detected among four sequences. But, BmHYII, BmHYIII and BmHYIV were short of stop-codon in the open reading frame and poly(A) signal in the 3' end.
透明质酸酶是蝎子毒液中的常见成分，被认为是一种“扩散因子”，可促进毒液在过敏反应中快速渗透。在本研究中，采用基于肽测序和快速扩增 cDNA 末端（RACE）的组合策略，克隆了一种新型全长透明质酸酶 BmHYI 和三种非编码同工型 BmHYII、BmHYIII 和 BmHYIV。BmHYI 含有 410 个氨基酸残基，包含催化、定位和五个潜在的 N-糖基化位点。BmHYI 的推导蛋白序列与来自蜜蜂和蛇的毒液透明质酸酶具有显著的同源性。系统发育分析表明，BmHYI 与其他透明质酸酶早期分化并独立进化。在四个序列之间检测到极高水平的序列相似性。但是，BmHYII、BmHYIII 和 BmHYIV 在开放阅读框中缺少终止密码子和 3' 端的聚（A）信号。
