羟基红花黄色素A对糖皮质激素诱导的骨髓间充质干细胞成骨分化的影响
[Effect of Hydroxy Safflower Yellow A on glucocorticoid-induced bone marrow mesenchymal stem cells osteogenic differentiation].
作者信息
Wan Tian, Wu Min-Rui, Qi Zhen-Xi
出版信息
Zhongguo Gu Shang. 2014 Mar;27(3):224-8.
OBJECTIVE
To observe the effect of Hydroxy Safflower Yellow A (HSYA) on the expression of osteogenic markers, such as alkaline phosphatase, Cbf(alpha)l and type I collagen, and explore the mechanism of HSYA in the prevention and treatment of glucocorticoid-induced ischemic necrosis of femoral head.
METHODS
Fifteen healthy and adult New Zealand white rabbits were collected and weighted 0.9 to 1.3 kg. The rabbits were injected abdominally with anesthetic drugs, then received marrow cavity puncture of tibia and anterior superior iliac spine to get bone marrow blood. Rabbits bone marrow mesenchymal stem cells (BMSCs) were separated from the bone marrow blood, cultured in vitro and passaged. The 3rd generation of BMSCs which had good growth condition were randomly divided into blank group, model group and HSYA groups with different doses. The BMSCs in model group were treated with high dose of dexamethasone to induce adipogenic differentiation of cells cultured in vitro, and inhibit osteogenic differentiation. The BMSCs in HSYA groups received high dose of dexamethasone and different concentrations of HSYA simultaneously. The blank group received not any special handling. After a week,the expressions of alkaline phosphatase, Cbf(alpha)l and type I collagen mRNA were detected.
RESULTS
The alkaline phosphatase activity was significantly decreased in BMSCs of the model group as compared with the blank group (P < 0.01), and the expression of Cbf(alpha)l and type I collagen mRNA were also decreased significantly (P<0.01). The alkaline phosphatase activity was significantly increased in BMSCs of each HSYA group as compared with the model group (P < 0.05 or P < 0.01), and the expression of Cbf(alpha)l and type I collagen mRNA were also increased significantly (P < 0.05 or P < 0.01).
CONCLUSION
The mechanism of HSYA may be related to the effect of antagonism to the reduced osteogenic differentiation induced by glucocorticoid.
目的
观察羟基红花黄色素A（HSYA）对碱性磷酸酶、Cbf（α）1和I型胶原等成骨标志物表达的影响，探讨HSYA防治糖皮质激素性股骨头缺血性坏死的作用机制。
方法
选取15只健康成年新西兰白兔，体重0.9～1.3 kg。兔腹部注射麻醉药后，行胫骨骨髓腔穿刺及髂前上棘骨髓穿刺获取骨髓血。从骨髓血中分离兔骨髓间充质干细胞（BMSCs），进行体外培养及传代。将生长状态良好的第3代BMSCs随机分为空白组、模型组和不同剂量HSYA组。模型组BMSCs用高剂量地塞米松处理，诱导体外培养细胞向脂肪分化，抑制成骨分化。HSYA组BMSCs同时接受高剂量地塞米松和不同浓度HSYA处理。空白组不做任何特殊处理。1周后，检测碱性磷酸酶、Cbf（α）1和I型胶原mRNA的表达。
结果
与空白组比较，模型组BMSCs碱性磷酸酶活性显著降低（P<0.01），Cbf（α）1和I型胶原mRNA表达也显著降低（P<0.01）。与模型组比较，各HSYA组BMSCs碱性磷酸酶活性显著升高（P<0.05或P<0.01），Cbf（α）1和I型胶原mRNA表达也显著升高（P<0.05或P<0.01）。
结论
HSYA的作用机制可能与拮抗糖皮质激素诱导的成骨分化降低有关。
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