Department of Gastroenterology and Hepatology, Royal Adelaide Hospital, Adelaide, South Australia, 5000, Australia; Discipline of Medicine, University of Adelaide, Royal Adelaide Hospital, Adelaide, South Australia, 5000, Australia.
Obesity (Silver Spring). 2014 Oct;22(10):2164-71. doi: 10.1002/oby.20829. Epub 2014 Jul 2.
To determine the effect of Roux-en-Y gastric bypass (RYGB) on the expression of intestinal sweet taste receptors (STRs), glucose transporters (GTs), glucose absorption, and glycemia.
Intestinal biopsies were collected for mRNA expression of STR (T1R2) and GTs (SGLT-1 and GLUT2) from 11 non-diabetic RYGB, 13 non-diabetic obese, and 11 healthy subjects, at baseline and following a 30 min small intestinal (SI) glucose infusion (30 g/150 ml water with 3 g 3-O-methyl-d-glucopyranose (3-OMG)). Blood glucose, plasma 3-OMG, and insulin were measured for 270 min.
In RYGB patients, expression of both GTs was ∼2-fold higher at baseline and after glucose infusion than those of morbidly obese or healthy subjects (P < 0.001). STR expressions were comparable amongst the groups. Peak plasma 3-OMG in both RYGB (r = 0.69, P = 0.01) and obese (r = 0.72, P = 0.005) correlated with baseline expression of SGLT-1, as was the case with peak blood glucose in RYGB subjects (r = 0.69, P = 0.02).
The upregulated intestinal GTs in RYGB patients are associated with increased glucose absorption when glucose is delivered at a physiological rate, suggesting a molecular adaptation to prevent carbohydrate malabsorption from rapid intestinal transit after RYGB.
确定 Roux-en-Y 胃旁路术（RYGB）对肠道甜味受体（STR）、葡萄糖转运体（GTs）、葡萄糖吸收和血糖的表达的影响。
收集 11 名非糖尿病 RYGB 患者、13 名非糖尿病肥胖患者和 11 名健康对照者的肠活检标本，检测 STR（T1R2）和 GTs（SGLT-1 和 GLUT2）的 mRNA 表达，基线时和 30 分钟后进行小肠（SI）葡萄糖输注（30 g/150 ml 水，含 3 g 3-O-甲基-d-吡喃葡萄糖（3-OMG））。在 270 分钟内测量血糖、血浆 3-OMG 和胰岛素。
在 RYGB 患者中，GTs 的表达在基线和葡萄糖输注后均比肥胖或健康对照组高约 2 倍（P < 0.001）。STR 表达在各组之间相似。RYGB（r = 0.69，P = 0.01）和肥胖组（r = 0.72，P = 0.005）的血浆 3-OMG 峰值与 SGLT-1 的基线表达相关，RYGB 受试者的血糖峰值也是如此（r = 0.69，P = 0.02）。
RYGB 患者肠道 GTs 的上调与生理速率下葡萄糖输送时葡萄糖吸收增加有关，这表明了一种分子适应，以防止 RYGB 后快速肠转运导致碳水化合物吸收不良。
