Miyagami M, Tazoe M, Kasahara E, Tsubokawa T
Dept. of Neurological Surgery, School of Medicine, Nihon University.
Gan To Kagaku Ryoho. 1989 May;16(5):2059-65.
The purpose of this study was to investigate the therapeutic effect of intracarotid infusion of 20% mannitol with ACNU chemotherapy in Fischer 344 rats with intracerebrally implanted 9L gliosarcoma, compared with giving them only ACNU intraperitoneally. Thirty 9L gliosarcoma bearing Fischer 344 rats were evaluated in the following 3 groups. Group I: control (no treatment); group II: treated by ACNU 20 mg/kg intraperitoneally on the 7th day after implantation of 9L gliosarcoma cells; group III: treated by intracarotid infusion of 20% mannitol 3.1 ml/min. and with the same dose of ACNU as in group II. Mean survival time after the inoculation of tumor cells into the brain was 15.1 days (group I), 21.8 days (group II) and 27.9 days (group III). In group II all tumor-bearing Fischer rats died within 24 days after inoculation of tumor cells, whereas in group III 5 out of 6 rats survived more than 25 days after them. Group III evidenced necrosis and degenerative findings with vacuole and microcyst without vascular proliferation in tumor tissues more than group II on the 7th day after ACNU treatment for the experimental brain tumor. On the 7th day after ACNU treatment of each group, BrdU labeling index was calculated in order to evaluate tumor proliferation. The Mean BrdU labeling index in tumor cells of group III demonstrated 7.5%, against 14.5% (about one half) in group II. From our experimental study of survival time and BrdU labeling index, it is suggested that osmotic blood brain barrier disruption chemotherapy by intracarotid infusion of 20% mannitol and ACNU was more effective than simple treatment by intraperitoneal injection of ACNU in the Fischer rats with intracerebrally implanted 9L gliosarcoma.
本研究的目的是调查在脑内植入9L胶质肉瘤的Fischer 344大鼠中，颈内动脉输注20%甘露醇联合ACNU化疗的治疗效果，并与仅腹腔注射ACNU进行比较。对30只携带9L胶质肉瘤的Fischer 344大鼠进行评估，并分为以下3组。第一组：对照组（未治疗）；第二组：在植入9L胶质肉瘤细胞后第7天，腹腔注射20mg/kg ACNU进行治疗；第三组：通过颈内动脉以3.1ml/min的速度输注20%甘露醇，并给予与第二组相同剂量的ACNU进行治疗。将肿瘤细胞接种到脑内后的平均生存时间，第一组为15.1天，第二组为21.8天，第三组为27.9天。在第二组中，所有携带肿瘤的Fischer大鼠在接种肿瘤细胞后24天内死亡，而在第三组中，6只大鼠中有5只在接种后存活超过25天。在对实验性脑肿瘤进行ACNU治疗后的第7天，第三组肿瘤组织中出现坏死和退行性改变，有液泡和微囊肿，无血管增生，比第二组更明显。在每组进行ACNU治疗后的第7天，计算BrdU标记指数以评估肿瘤增殖情况。第三组肿瘤细胞中的平均BrdU标记指数为7.5%，而第二组为14.5%（约为一半）。从我们关于生存时间和BrdU标记指数的实验研究来看，对于脑内植入9L胶质肉瘤的Fischer大鼠，颈内动脉输注20%甘露醇联合ACNU的渗透性血脑屏障破坏化疗比单纯腹腔注射ACNU治疗更有效。
