Shilling F, Chiba K, Hoshi M, Kishimoto T, Jaffe L A
Department of Biology, University of Southern California, Los Angeles 90089.
Dev Biol. 1989 Jun;133(2):605-8. doi: 10.1016/0012-1606(89)90063-8.
Starfish oocytes injected with pertussis toxin (3-6 micrograms/ml) or its catalytically active A-subunit (1 microgram/ml) did not undergo germinal vesicle breakdown in response to 1-methyladenine (1-10 microM). The pertussis block could be bypassed by transfer of cytoplasm that contained maturation-promoting factor (MPF). After insemination, pertussis-blocked, MPF-rescued oocytes underwent cortical vesicle exocytosis and cleavage. These results suggest the involvement of a pertussis sensitive G-protein in the pathway coupling 1-methyladenine action at the cell surface to the reinitiation of meiosis.
注射百日咳毒素（3 - 6微克/毫升）或其催化活性A亚基（1微克/毫升）的海星卵母细胞，对1 - 甲基腺嘌呤（1 - 10微摩尔）无反应，不会发生生发泡破裂。含有成熟促进因子（MPF）的细胞质转移可绕过百日咳毒素的阻断作用。受精后，受百日咳毒素阻断但经MPF拯救的卵母细胞会发生皮质囊泡胞吐作用和卵裂。这些结果表明，一种对百日咳毒素敏感的G蛋白参与了将细胞表面1 - 甲基腺嘌呤作用与减数分裂重新启动相偶联的信号通路。
