Santos-Gandelman Juliana F, Giambiagi-deMarval Marcia, Muricy Guilherme, Barkay Tamar, Laport Marinella S
Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Av. Carlos Chagas Filho, 373, Cidade Universitária, Rio de Janeiro, 21941-902, Brazil.
Antonie Van Leeuwenhoek. 2014 Sep;106(3):585-90. doi: 10.1007/s10482-014-0224-2. Epub 2014 Jul 5.
Ionic and organic forms of mercury (Hg) are powerful cytotoxic and neurotoxic agents in both humans and wild life. The aim of this study was to analyze the resistance profile and potential detoxification of inorganic and organic forms of Hg of bacteria isolated from marine sponges on the coast of Rio de Janeiro, Brazil. Out of the 1,236 colony forming units associated with eleven species of marine sponges, 100 morphologically different bacterial strains were analyzed in this study. Of these, 21 strains were resistant to Hg, 14 of which were classified as highly resistant because they grew despite exposure to 100 µM HgCl2. Fifteen resistant strains reduced Hg and presented merA in their genomes. The remaining six strains produced biosurfactants, suggesting that they may tolerate Hg by sequestration. Eleven strains grew in the presence of methylmercury. Our results suggest a potential for mercury detoxification by marine sponge-associated resistant bacteria, either through reduction or sequestration, as well as the possibility of bioremediation of toxic waste containing mercury.
汞（Hg）的离子形式和有机形式对人类和野生动物都是强大的细胞毒性和神经毒性剂。本研究的目的是分析从巴西里约热内卢海岸的海洋海绵中分离出的细菌对无机汞和有机汞的抗性谱及潜在解毒作用。在与11种海洋海绵相关的1236个菌落形成单位中，本研究分析了100株形态不同的细菌菌株。其中，21株对汞具有抗性，其中14株被归类为高度抗性，因为它们在暴露于100μM HgCl2的情况下仍能生长。15株抗性菌株能还原汞且其基因组中存在merA。其余6株产生生物表面活性剂，表明它们可能通过螯合作用耐受汞。11株在甲基汞存在的情况下生长。我们的结果表明，海洋海绵相关抗性细菌具有通过还原或螯合作用进行汞解毒的潜力，以及对含汞有毒废物进行生物修复的可能性。
