储存对基质利用与微生物生长之间呼吸计量关系的影响。
Effect of storage on the respirometric relationship between substrate utilization and microbial growth.
机构信息
Department of Environmental Engineering, Akdeniz University, Antalya, Turkey.
Environmental Engineering Department, Istanbul Technical University, 34469 Maslak, Istanbul, Turkey; ENVIS Energy and Environmental Systems R&D Ltd, ITU Arı Teknokent, Arı 1 Building No. 16, Maslak 34469, Istanbul, Turkey.
出版信息
Bioresour Technol. 2014 Sep;167:331-5. doi: 10.1016/j.biortech.2014.06.039. Epub 2014 Jun 18.
The paper evaluated the impact of substrate storage on the respirometric assessment of process stoichiometry based on oxygen uptake rate (OUR) measurements. Two parallel sequencing batch reactors were operated with pulse feeding of synthetic substrate mixture at a sludge age of 8 days and 2 days. During the cycle experiments with acetate, 40-45% of acetate was converted to polyhydroxybutyrate, which was partly consumed during each cycle. Respirometric analysis also yielded OUR profiles for the corresponding cyclic operation. A mass balance expression was derived based on oxygen utilization. Oxygen demands calculated on the basis of partial PHB utilization closely matched the experimental values retrieved from OUR profiles within limits of analytical precision. The relative contribution of storage mechanism represented more than 50% of overall oxygen demand. Substrate storage, when totally disregarded or not properly evaluated, was observed to involve an error of around 10% on overall the oxygen demand.
本文评估了基于耗氧速率（OUR）测量的底物储存对工艺化学计量比呼吸计量评估的影响。采用合成底物混合物脉冲进料，在污泥龄为 8 天和 2 天的条件下，平行运行了两个序批式反应器。在乙酸的循环实验中，40-45%的乙酸转化为聚羟基丁酸（PHB），在每个循环中部分被消耗。呼吸计量分析也得到了相应的循环运行的 OUR 曲线。基于氧利用，推导出了一个质量平衡表达式。基于部分 PHB 利用计算的氧需求与从 OUR 曲线中检索到的实验值在分析精度范围内非常吻合。储存机制的相对贡献超过总需氧量的 50%。当完全忽略或未正确评估底物储存时，观察到总需氧量存在约 10%的误差。
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