Lee Sanghee, Yang Guang, Mulligan William, Gipp Jerry, Bushman Wade
Department of Urology, University of Wisconsin School of Medicine and Public Health, Madison, WI 53717, USA ; Cellular and Molecular Biology Program, University of Wisconsin-Madison School of Medicine and Public Health, Madison, WI 53706, USA.
Department of Urology, University of Wisconsin School of Medicine and Public Health, Madison, WI 53717, USA.
J Diabetes Res. 2014;2014:939053. doi: 10.1155/2014/939053. Epub 2014 Jun 11.
A higher incidence of lower urinary tract symptoms (LUTS) among diabetic men is unexplained. Recently, prostate inflammation and fibrosis have been implicated as major contributing factors to bladder outlet obstruction and LUTS. We characterized the inflammatory cell infiltrate and collagen content of the anterior, dorsal, and ventral lobes of 18-week-old DBA2J.Ins2-Akita mice (Akita) and age-matched control mice. We performed hematoxylin and eosin staining to score tissue injury and inflammation, picrosirius red staining to quantitate collagen content, and immunostaining to identify monocytes/macrophages and infiltrating fibrocytes. We observed significantly greater numbers of monocytes/macrophages and fibrocytes specifically in the ventral prostate of the Akita mice and found that this was associated with significant greater collagen content specifically in the ventral prostate of the Akita mice. These observations support the inference that diabetes elicits monocyte/macrophage infiltration and collagen accumulation in the prostate and suggest that further study of Akita mice may inform translational studies of diabetes in the genesis prostatic inflammation, prostatic fibrosis, and LUTS.
糖尿病男性下尿路症状（LUTS）的发病率较高，原因不明。最近，前列腺炎症和纤维化被认为是导致膀胱出口梗阻和LUTS的主要因素。我们对18周龄的DBA2J.Ins2-Akita小鼠（秋田小鼠）和年龄匹配的对照小鼠的前列腺前叶、背叶和腹叶的炎性细胞浸润和胶原蛋白含量进行了表征。我们进行了苏木精和伊红染色以对组织损伤和炎症进行评分，进行了天狼星红苦味酸染色以定量胶原蛋白含量，并进行了免疫染色以识别单核细胞/巨噬细胞和浸润的纤维细胞。我们观察到，秋田小鼠的腹侧前列腺中单核细胞/巨噬细胞和纤维细胞的数量明显更多，并且发现这与秋田小鼠腹侧前列腺中明显更高的胶原蛋白含量有关。这些观察结果支持了糖尿病引发前列腺中单核细胞/巨噬细胞浸润和胶原蛋白积累的推断，并表明对秋田小鼠的进一步研究可能为糖尿病在前列腺炎症、前列腺纤维化和LUTS发生中的转化研究提供信息。
