Key Laboratory of Acupuncture-Moxibustion and Immunological Effects, Shanghai University of Traditional Chinese Medicine, Shanghai 200030, China.
Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China.
Evid Based Complement Alternat Med. 2014;2014:807308. doi: 10.1155/2014/807308. Epub 2014 Jun 12.
It has been proven that prokineticin 2 (PK2) and its receptor PKR2 play an important role in hyperalgesia, while mild moxibustion can relieve visceral hypersensitivity in a rat model of irritable bowel syndrome (IBS). The goal of the present study was to determine the effects of mild moxibustion on the expression of PK2 and PKR2 in colon and spinal cord in IBS rat model, which was induced by colorectal distension using inflatable balloons. After mild moxibustion treatment, abdominal withdrawal reflex (AWR) scores were assessed by colorectal distension; protein and mRNA expression of PK2 and PKR2 in rat colon and spinal cord was determined by immunohistochemistry and fluorescence quantitative PCR. Compared with normal rats, the AWR scores of rats and the expressions of PK2/PKR2 proteins and mRNAs in colon and spinal cord tissue were significantly increased in the model group; compared with the model group, the AWR scores of rats and the expressions of PK2/PKR2 proteins and mRNAs in colon and spinal cord tissue were significantly decreased in the mild moxibustion group. These findings suggest that the analgesia effect of mild moxibustion may be associated with the reduction of the abnormally increased expression of the PK2/PKR2 proteins and mRNAs in the colon and spinal cord.
已经证明,促动力素 2(PK2)及其受体 PKR2 在痛觉过敏中发挥重要作用,而温和灸可以缓解肠易激综合征(IBS)大鼠模型中的内脏高敏感性。本研究的目的是确定温和灸对充气球扩张诱导的 IBS 大鼠模型结肠和脊髓中 PK2 和 PKR2 表达的影响。温和灸治疗后,通过结肠扩张评估腹壁退缩反射(AWR)评分;通过免疫组织化学和荧光定量 PCR 测定大鼠结肠和脊髓中 PK2 和 PKR2 蛋白和 mRNA 的表达。与正常大鼠相比,模型组大鼠的 AWR 评分以及结肠和脊髓组织中 PK2/PKR2 蛋白和 mRNA 的表达均显著增加;与模型组相比,温和灸组大鼠的 AWR 评分以及结肠和脊髓组织中 PK2/PKR2 蛋白和 mRNA 的表达均显著降低。这些发现表明,温和灸的镇痛作用可能与结肠和脊髓中 PK2/PKR2 蛋白和 mRNA 的异常高表达的减少有关。